PXD023876

PXD023876 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of HCC-1954 and MCF-7 cell lines highlights crosstalk between αvβ1 integrin, E-cadherin and HER-2
Description	Overexpression of human epidermal growth factor receptor 2 (HER-2) occurs in 20% of all breast cancer subtypes, those that present the worst prognostic outcome through a very invasive and aggressive tumour. HCC-1954 (HER-2+) is a highly invasive, metastatic cell line whereas MCF-7 is mildly aggressive and non-invasive. We investigated membrane proteins from both cell lines that could have a pivotal biological significance in the metastatic process. Membrane protein enrichment for HCC-1954 and MCF-7 proteomic analysis was performed. The samples were analysed on a two-dimensional liquid chromatography coupled to mass spectrometry (2-D-SCX/RP-LCMS system) and the protein expression was quantified by MSE method. High abundance membrane proteins were confirmed by western blot, immunofluorescence, and flow cytometry. Protein interaction prediction and correlations with TCGA patient’s data were conducted by bioinformatic analysis. The comparison between HCC-1954 and MCF-7 membrane proteins revealed that proteins involved in cytoskeleton organization, such as HER-2, β-1 integrin, E-cadherin and CD166 (ALCAM) were more abundant in HCC-1954. The Cancer Genome Atlas (TCGA) analysis showed a trend toward a positive correlation between HER-2 and β-1 integrin in HER-2+ breast cancer patients. Differences in protein profile and abundance reflect distinctive capabilities for motility and invasiveness between cell lines. HCC-1954 could be an excellent model to study β1 integrin and epithelial–mesenchymal transition (EMT) involvement in trastuzumab resistance mechanisms.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:41:35.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Denise de Abreu Pereira
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; amidated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-29 01:29:26	ID requested
1	2022-10-13 17:53:18	announced
⏵ 2	2023-11-14 07:41:39	announced	2023-11-14: Updated project metadata.

de Abreu Pereira D, Sandim V, Fernandes TFB, Almeida VH, Rocha MR, do Amaral RJFC, Rossi MID, Kalume DE, Zingali RB, 1 Integrins, E-Cadherin and HER-2. Int J Mol Sci, 23(17):(2022) [pubmed]

submitter keyword: HCC-1954, membrane proteins, HER-2,Breast cancer, MCF-7, proteome

Russolina B. Zingali
contact affiliation	Unidade de Espectrometria de Massas e Proteômica (UEMP) Programa de Biologia Estrutural Bloco D, Primeiro andar, Sala 45 – Centro de ciências da saúde - Universidade Federal do Rio de Janeiro
contact email	lzingali@bioqmed.ufrj.br
lab head
Denise de Abreu Pereira
contact affiliation	National Instituit of Cancer from Rio de Janeiro
contact email	deniseap@inca.gov.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD023876

PRIDE project URI

• PRIDE
  1. PXD023876
     1. Label: PRIDE project
     2. Name: Proteomic analysis of HCC-1954 and MCF-7 cell lines highlights crosstalk between αvβ1 integrin, E-cadherin and HER-2