PXD023874

PXD023874 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Malic enzyme 2 mediates fumarate signaling to regulate mitochondrial biogenesis
Description	Mitochondria have an independent genome (mtDNA) and protein synthesis machinery that coordinately activate for mitochondrial generation. Here, we report that Krebs cycle intermediate, fumarate, connects metabolism to mitobiogenesis through binding to malic enzyme 2 (ME2). Proteomic profiling of ME2-interacting protein reveals that ME2 binds to mitoribosome proteins to regulate mitoribosome assembly and mtDNA-encoded protein production. ME2 also interacts with and modulates deoxyuridine 5-triphosphate nucleotidohydrolase activity to regulate thymidine generation and mtDNA abundance
HostingRepository	MassIVE
AnnounceDate	2021-01-29
AnnouncementXML	Submission_2021-01-29_14:11:11.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bogdan Budnik
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Deamidated; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-28 12:26:56	ID requested
⏵ 1	2021-01-29 14:11:12	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Malic enzyme 2, Fumarate, Deoxyuridine 5-triphosphate nucleotidohydrolase, Mitochondrial ribosome protein L45, Mitochondrial ribosome

submitter keyword: Malic enzyme 2, Fumarate, Deoxyuridine 5-triphosphate nucleotidohydrolase, Mitochondrial ribosome protein L45, Mitochondrial ribosome

Contact List

David Scadden
contact affiliation	Department of Stem Cell and Regenerative Biology, Harvard University
contact email	dscadden@mgh.harvard.edu
lab head
Bogdan Budnik
contact affiliation	Harvard University
contact email	bbudnik@mcb.harvard.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086778/