PXD023872 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of the MuRF1 skeletal muscle ubiquitylome through quantitative proteomics |
Description | MuRF1 is a muscle-specific E3 ubiquitin ligase and component of the ubiquitin proteasome system. MuRF1 is transcriptionally upregulated under conditions that cause muscle loss, in both rodents and humans, and is a recognized marker of muscle atrophy. In this study, we used in vivo electroporation to determine if MuRF1 overexpression alone can cause muscle atrophy and, in combination with ubiquitin proteomics, identify the endogenous MuRF1 substrates in skeletal muscle. Tibialis anterior (TA) muscles were transfected with an untagged MuRF1 plasmid or control plasmid for 14 days. A total of 963 ubiquitination sites, corresponding to 250 proteins, were quantified from the TA muscle. Statistical analysis revealed that the overexpression of MuRF1 resulted in significant upregulation of 153 ubiquitination sites on 45 proteins and significant downregulation of 16 sites on 11 proteins. Substrates of MuRF1 include contractile and metabolic proteins, deubiquitinases, p62, and VCP. Moreover, MuRF1-mediated ubiquitination leads to destabilization and breakdown of the sarcomere and reveals a role for MuRF1 in the regulation of additional proteolytic pathways in skeletal muscle. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-07 |
AnnouncementXML | Submission_2021-07-06_23:09:49.807.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Delphi Van Haver |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | ubiquitination signature dipeptidyl lysine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-28 09:03:16 | ID requested | |
⏵ 1 | 2021-07-06 23:09:50 | announced | |
Publication List
Baehr LM, Hughes DC, Lynch SA, Van Haver D, Maia TM, Marshall AG, Radoshevich L, Impens F, Waddell DS, Bodine SC, Identification of the MuRF1 Skeletal Muscle Ubiquitylome Through Quantitative Proteomics. Function (Oxf), 2(4):zqab029(2021) [pubmed] |
Keyword List
submitter keyword: muscle atrophy, MuRF1, electroporation, ubiquitin proteomics, protein degradation |
Contact List
Sue Bodine |
contact affiliation | Department of Internal Medicine, Division of Endocrinology and Metabolism, Carver College of Medicine, University of Iowa, Iowa City, Iowa, 52242 USA |
contact email | sue-bodine@uiowa.edu |
lab head | |
Delphi Van Haver |
contact affiliation | VIB Proteomics Core |
contact email | delphi.vanhaver@vib-ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023872
- Label: PRIDE project
- Name: Identification of the MuRF1 skeletal muscle ubiquitylome through quantitative proteomics