PXD023862

PXD023862 is an original dataset announced via ProteomeXchange.

Title	SSc biomarkers detection in the secretome of TGF1-activated primary human lung fibroblasts.
Description	TGFβ1 is a profibrotic mediator that contributes to a broad spectrum of pathologies, including pulmonary fibrosis (PF). However, the secretome of TGFβ1-stimulated primary human normal lung (NL) fibroblasts has not been well characterized. Using fluorescent 2-dimensional gel electrophoresis (2D-PAGE) and differential gel electrophoresis (DIGE), we identified 37 differentially secreted proteins in the conditioned media of TGFβ1-activated NL fibroblasts and generated a protein-protein association network of the TGFβ1 secretome using STRING. Functional enrichment revealed that biological processes and pathways characteristics of PF were enriched. Using the DrugBank database, we determined that 32 of the secreted proteins are targets of known experimental, investigational and approved drugs. Additionally, by comparing the TGFβ1 secretome of NL fibroblasts to proteomic biomarkers from biological fluids of systemic sclerosis (SSc) patients, we identified 11 overlapping proteins. Together our data validate the TGFβ1-induced secretome of NL fibroblasts as a valid in vitro model that reflects SSc biomarkers and identifies potential therapeutic targets for SSc-PF.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:53:40.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Bethard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-27 22:41:40	ID requested
⏵ 1	2022-08-11 23:53:41	announced

Kendall RT, Renaud L, Baatz JE, Malaab M, Nguyen XX, Feghali-Bostwick CA, 1-activated primary human lung fibroblasts. J Proteomics, 242():104243(2021) [pubmed]

submitter keyword: Fibroblasts, TGFbeta, Secretome

Carol A. Feghali-Bostwick
contact affiliation	Medical University of South Carolina, Department of Medicine, Division of Rheumatology & Immunology, 96 Jonathan Lucas St, MSC 637, Charleston, SC, 29425, United State of America.
contact email	feghalib@musc.edu
lab head
Jennifer Bethard
contact affiliation	Medical University of SC
contact email	bethard@musc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD023862

PRIDE project URI

• PRIDE
  1. PXD023862
     1. Label: PRIDE project
     2. Name: SSc biomarkers detection in the secretome of TGF1-activated primary human lung fibroblasts.