PXD023860

PXD023860 is an original dataset announced via ProteomeXchange.

Title	Mouse hepatocyte-derived extracellular vesicle proteome analysis
Description	Mouse hepatocyte AML12 cells were plated in T175 flasks until they reached >90% confluency after which spent medium was removed, and the cells were rinsed twice with Hanks Balanced Salt Solution (ThermoFisher, Waltham, MA, United States) prior to incubating them in serum-free medium overnight, followed by replacement with fresh serum-free medium for 48 h. The supernatants were subjected to sequential centrifugation (300 × g for 10 min, 2,000 × g for 20 min, 10,000 × g for 30 min) and ultracentrifugation (100,000 × g for 70 min at 4◦C) in a Type T70i fixed-angle rotor, the pellet from which was resuspended and subjected to the same ultracentrifugation conditions again. The resulting EV pellet was dispersed in PBS, followed by protein concentration measurement by BCA assay. 5 ug of proteins were sent for mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-10_00:15:35.573.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinlei Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-27 22:36:26	ID requested
⏵ 1	2021-09-10 00:15:36	announced

Li X, Chen R, Kemper S, Brigstock DR, Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes. Front Cell Dev Biol, 9():640667(2021) [pubmed]

submitter keyword: mouse hepatocytes, extracellular vesicles, proteome

David R Brigstock
contact affiliation	The Abigail Wexner Research Institute at Nationwide Children’s Hospital
contact email	David.Brigstock@nationwidechildrens.org
lab head
Xinlei Li
contact affiliation	Nationwide Children's Hospital
contact email	xinlei.li@nationwidechildrens.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023860

PRIDE project URI

• PRIDE
  1. PXD023860
     1. Label: PRIDE project
     2. Name: Mouse hepatocyte-derived extracellular vesicle proteome analysis