PXD023846 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Preserved respiratory chain capacity and physiology in mice with profoundly reduced levels of mitochondrial respirasomes |
Description | The mammalian respiratory chain complexes I, III2 and IV (CI, CIII2 and CIV) are critical for cellular bioenergetics and form a stable assembly, the respirasome (CI-CIII2-CIV), that is biochemically and structurally well documented. The role of the respirasome in bioenergetics and regulation of metabolism is subject to intense debate and is difficult to study because the individual respiratory chain complexes coexist together with high levels of respirasomes. To critically investigate the in vivo role of the respirasome, we generated homozygous knock-in mice that have normal levels of respiratory chain complexes but profoundly decreased levels of respirasomes. Surprisingly, the mutant mice are healthy, with preserved respiratory chain capacity and normal exercise performance. Our findings show that high levels of respirasomes are dispensable for maintaining bioenergetics and physiology in the mouse, but raises questions about their alternate functions, such as relating to regulation of protein stability and prevention of age-associated protein aggregation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-19 |
AnnouncementXML | Submission_2024-03-19_07:04:10.848.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ilian Atanassov |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-26 23:26:59 | ID requested | |
⏵ 1 | 2024-03-19 07:04:11 | announced | |
Publication List
Milenkovic D, Misic J, Hevler JF, Molini, é T, Chung I, Atanassov I, Li X, Filograna R, Mesaros A, Mourier A, Heck AJR, Hirst J, Larsson NG, Preserved respiratory chain capacity and physiology in mice with profoundly reduced levels of mitochondrial respirasomes. Cell Metab, 35(10):1799-1813.e7(2023) [pubmed] |
10.1016/j.cmet.2023.07.015; |
Keyword List
submitter keyword: TMT, supercomplex,OXPHOS, mitochondria, complexome profiling |
Contact List
Nils-Göran Larsson |
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden |
contact email | nils-goran.larsson@ki.se |
lab head | |
Ilian Atanassov |
contact affiliation | Max Planck Institute for Biology of Aging |
contact email | Ilian.Atanassov@age.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023846
- Label: PRIDE project
- Name: Preserved respiratory chain capacity and physiology in mice with profoundly reduced levels of mitochondrial respirasomes