PXD023846

PXD023846 is an original dataset announced via ProteomeXchange.

Title	Preserved respiratory chain capacity and physiology in mice with profoundly reduced levels of mitochondrial respirasomes
Description	The mammalian respiratory chain complexes I, III2 and IV (CI, CIII2 and CIV) are critical for cellular bioenergetics and form a stable assembly, the respirasome (CI-CIII2-CIV), that is biochemically and structurally well documented. The role of the respirasome in bioenergetics and regulation of metabolism is subject to intense debate and is difficult to study because the individual respiratory chain complexes coexist together with high levels of respirasomes. To critically investigate the in vivo role of the respirasome, we generated homozygous knock-in mice that have normal levels of respiratory chain complexes but profoundly decreased levels of respirasomes. Surprisingly, the mutant mice are healthy, with preserved respiratory chain capacity and normal exercise performance. Our findings show that high levels of respirasomes are dispensable for maintaining bioenergetics and physiology in the mouse, but raises questions about their alternate functions, such as relating to regulation of protein stability and prevention of age-associated protein aggregation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:34:07.843.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 23:26:59	ID requested
1	2024-03-19 07:04:11	announced
⏵ 2	2024-10-22 06:34:08	announced	2024-10-22: Updated project metadata.

Milenkovic D, Misic J, Hevler JF, Molini, é T, Chung I, Atanassov I, Li X, Filograna R, Mesaros A, Mourier A, Heck AJR, Hirst J, Larsson NG, Preserved respiratory chain capacity and physiology in mice with profoundly reduced levels of mitochondrial respirasomes. Cell Metab, 35(10):1799-1813.e7(2023) [pubmed]

10.1016/j.cmet.2023.07.015;

submitter keyword: TMT, supercomplex,OXPHOS, mitochondria, complexome profiling

Nils-Göran Larsson
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden
contact email	nils-goran.larsson@ki.se
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD023846

PRIDE project URI

• PRIDE
  1. PXD023846
     1. Label: PRIDE project
     2. Name: Preserved respiratory chain capacity and physiology in mice with profoundly reduced levels of mitochondrial respirasomes