PXD023831

PXD023831 is an original dataset announced via ProteomeXchange.

Title	Post-translational modification mapping - CRK10 WT and crk10-A397T kinase domain
Description	We have analysed the recombinant kinase domain of AtCRK10 (At4g23180) purified as an N-terminal His-tag from Escherichia coli cells by LC-MS/MS. Both the WT and the crk10-A39T7 mutant allele of the CRK10 kinase domain were analysed. Post-translational modifications were identified, and a MASCOT search was carried out.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:13.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maiara Piovesana
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 04:34:30	ID requested
1	2023-07-20 10:34:56	announced
⏵ 2	2023-11-14 09:02:14	announced	2023-11-14: Updated project metadata.

Piovesana M, Wood AKM, Smith DP, Deery MJ, Bayliss R, Carrera E, Wellner N, Kosik O, Napier JA, Kurup S, Matthes MC, A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis. J Exp Bot, 74(10):3104-3121(2023) [pubmed]

submitter keyword: Arabidopsis, cysteine-rich receptor-like kinase,Kinase domain, CRK10

Michaela Matthes
contact affiliation	Department of Plant Sciences Rothamsted Research West Common Harpenden AL5 2JQ UK
contact email	michaela.matthes@rothamsted.ac.uk
lab head
Maiara Piovesana
contact affiliation	Rothamsted Research
contact email	maiara.piovesana@rothamsted.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD023831

PRIDE project URI

• PRIDE
  1. PXD023831
     1. Label: PRIDE project
     2. Name: Post-translational modification mapping - CRK10 WT and crk10-A397T kinase domain