PXD023830

PXD023830 is an original dataset announced via ProteomeXchange.

Title	Changes in Caenorhabditis elegans proteome after dnj-21 knockdown
Description	Dysfunction of the motor subunit of the TIM23 translocase, the PAM complex located on the matrix side of the mitochondrial inner membrane in Saccharomyces cerevisiae, was shown to cause a decrease in mitochondrial protein import and precursor accumulation in the cytosol. We used an analogous model to study the non-mitochondrial response to defective mitochondrial import machinery in Caenorhabditis elegans in which we depleted DNJ-21 as the functional homolog of yeast Pam18. To gain a broader insight in potential changes in Caenorhabditis elegans proteome upon DNJ-21 depletion we performed a quantitative, label-free proteomics analysis. We compared protein levels upon knockdown of dnj-21 (dnj-21 RNAi) with control conditions (Empty vector RNAi). Synchronized N2 wild type worms were grown on NGM plates seeded with E. coli HT115(DE3) transformed with a construct targeting dnj-21 gene or with empty vector L4440 as a control.
HostingRepository	PRIDE
AnnounceDate	2021-05-19
AnnouncementXML	Submission_2021-05-19_07:46:09.800.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 04:34:26	ID requested
⏵ 1	2021-05-19 07:46:10	announced

Dataset with its publication pending

submitter keyword: Caenorhabditis elegans, dnj-21 knockdown, mitochondria, mitochondrial stress

Agnieszka Chacinska
contact affiliation	ReMedy International Research Agenda Unit, University of Warsaw, Warsaw 02-097, Poland; Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland
contact email	a.chacinska@cent.uw.edu.pl
lab head
Remigiusz Serwa
contact affiliation	Centre of New Technologies, University of Warsaw
contact email	r.serwa@cent.uw.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD023830

PRIDE project URI

• PRIDE
  1. PXD023830
     1. Label: PRIDE project
     2. Name: Changes in Caenorhabditis elegans proteome after dnj-21 knockdown