PXD023826

PXD023826 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis in transgenic hybrid aspen (Populus tremula x tremuloides) trees overexpression of vesicle-associated membrane protein PttVAP27-17
Description	In this study we report on transgenic hybrid aspen (Populus tremula x P. tremuloides) lines that showed potential to increase biomass production both in the greenhouse and after five years of growth in the field. The transgenic lines carried an overexpression construct for Populus tremula x tremuloides vesicle-associated membrane protein (VAMP)-associated protein PttVAP27-17 (Potri.019G116400) that was selected from a gene-mining program for novel regulators of wood formation. A proteomic analysis was performed to characterize the overall effect of the overexpression of PttVAP27-17 on plant metabolic pathways. 20 mg of xylem sample for the selected wild type (WT) and three transgenic lines (lines 1,2 and 3) was collected in the following manner from two-months-old, greenhouse grown trees: The frozen bottom-part of the stem (10-17 cm portion from the base of the stem) was peeled, and the surface of the secondary xylem consisting of living vessels and fibers (into the depth of approximately one mm from the surface) was scraped. The xylem scrapings were ground to fine powder in liquid nitrogen and stored at -80oC. The analyses included 3-5 biological replicates for each of the transgenic lines, and seven replicates for the wild type.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-10_00:16:19.192.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joakim Bygdell
SpeciesList	scientific name: Populus tremula x Populus tremuloides; NCBI TaxID: 47664;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 02:17:33	ID requested
⏵ 1	2021-09-10 00:16:19	announced

Gandla ML, M, ä, hler N, Escamez S, Skotare T, Obudulu O, M, ö, ller L, Abreu IN, Bygdell J, Hertzberg M, Hvidsten TR, Moritz T, Wingsle G, Trygg J, Tuominen H, J, ö, nsson LJ, Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees. Biotechnol Biofuels, 14(1):43(2021) [pubmed]

submitter keyword: Wood, Populus, HDMSE,

Hannele Tuominen
contact affiliation	Umea Plant Science Centre, Department of Forest Genetics & Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83 Umea, Sweden
contact email	hannele.tuominen@slu.se
lab head
Joakim Bygdell
contact affiliation	Computational Life Science Cluster (CLiC), Deptartment of Chemistry, Umeå University
contact email	joakim.bygdell@umu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023826
     1. Label: PRIDE project
     2. Name: Proteomic analysis in transgenic hybrid aspen (Populus tremula x tremuloides) trees overexpression of vesicle-associated membrane protein PttVAP27-17