PXD023818

PXD023818 is an original dataset announced via ProteomeXchange.

Title	Label-Free Phosphoproteomics Profiling of Fission Yeast Schizosaccharomyces pombe
Description	The phosphorylation of proteins modulates various functions of proteins and plays an important role in regulation of cell signaling. In the recent years, the label-free quantitative (LFQ) phos-phoproteomics has become the powerful tool to analyze the phosphorylation of proteins within the complex samples. Despite the great progress, the studies of protein phosphorylations are still limited in throughput, robustness, and reproducibility, hampering analyses that involve multiple perturbations, such as those needed to follow the dynamics of phosphoproteomes. To address these challenges, we introduce here the LFQ phosphoproteomics workflow that is based on Fe-IMAC phosphopeptide enrichment followed by strong anion exchange (SAX) and porous graphitic carbon (PGC) fractionation strategies. We applied this workflow to analyze the whole-cell phosphoproteome of the fission yeast Schizosaccharomyces pombe. Using the strategy, we identified 8353 phosphosites from which 1274 were newly identified. This provides the sig-nificant addition to the S. pombe phosphoproteome. Results of our study highlight that combining of PGC and SAX fractionation strategies substantially increases the robustness and specificity of LFQ phosphoproteomics. Overall, the presented LFQ phosphoproteomics workflow opens the door for studies that would get better insight into the complexity of the protein kinase functions of the fission yeast S. pombe.
HostingRepository	PRIDE
AnnounceDate	2021-02-12
AnnouncementXML	Submission_2021-02-12_04:36:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barath
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 01:41:19	ID requested
⏵ 1	2021-02-12 04:36:31	announced

Sivakova B, Jurcik J, Lukacova V, Selicky T, Cipakova I, Barath P, Cipak L, Using Strong Anion Exchange- and Porous Graphitic Carbon-Based Fractionation Strategies. Int J Mol Sci, 22(4):(2021) [pubmed]

submitter keyword: LFQ phosphoproteomics, SAX, PGC, Schizosaccharomyces pombe

Peter Barath
contact affiliation	Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
contact email	peter.barath@savba.sk
lab head
Peter Barath
contact affiliation	Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023818
     1. Label: PRIDE project
     2. Name: Label-Free Phosphoproteomics Profiling of Fission Yeast Schizosaccharomyces pombe