PXD023816

PXD023816 is an original dataset announced via ProteomeXchange.

Title	The fungivorous phagocyte Protostelium aurantium targets redox homeostasis and cell wall integrity during intracellular killing of Candida parapsilosis
Description	Predatory interactions among microbes are a major evolutionary driving force for biodiversity. The fungivorous amoeba Protostelium aurantium has a wide fungal food spectrum including major pathogenic members of the genus Candida. Phagocytic feeding by P. aurantium is highly effective with C. parapsilosis, a major pathogenic yeast. Here we show that upon ingestion by the amoeba C. parapsilosis is confronted with an oxidative burst and undergoes phagosomal lysis within minutes. On the fungal side, a functional genomic approach identified the fungal copper and redox homeostasis as primary targets of amoeba predation with the highly expressed copper exporter Crp1 and the peroxiredoxin Prx1 contributing to survival when encountering P. aurantium. The fungolytic activity was largely retained in intracellular vesicles of the amoebae. Following their isolation, the content of these vesicles induced immediate killing and lysis of C. parapsilosis. A proteomic analyses identified 56 vesicular proteins. Although fully unknown proteins were dominant, many of them could be categorized as hydrolytic enzymes presumably targeting the fungal cell wall, indicating that fungal cell wall structures are under predatory selection pressure in natural environments.
HostingRepository	PRIDE
AnnounceDate	2021-12-01
AnnouncementXML	Submission_2021-12-01_06:31:27.888.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Planoprotostelium fungivorum; NCBI TaxID: 1890364; scientific name: Candida parapsilosis; NCBI TaxID: 5480;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 01:41:05	ID requested
⏵ 1	2021-12-01 06:31:29	announced

Radosa S, Sprague JL, Lau SH, T, ó, th R, Linde J, Kr, ü, ger T, Sprenger M, Kasper L, Westermann M, Kniemeyer O, Hube B, Brakhage AA, G, á, cser A, Hillmann F, The fungivorous amoeba Protostelium aurantium targets redox homeostasis and cell wall integrity during intracellular killing of Candida parapsilosis. Cell Microbiol, 23(11):e13389(2021) [pubmed]

submitter keyword: LC-MS/MS, amoeba, Protostelium, Candida parapsilosis, predation, redox homeostasis

Axel A. Brakhage
contact affiliation	Molecular and Applied Microbiology Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute Adolf-Reichwein-Str. 23, 07745, Jena, Germany
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023816
     1. Label: PRIDE project
     2. Name: The fungivorous phagocyte Protostelium aurantium targets redox homeostasis and cell wall integrity during intracellular killing of Candida parapsilosis