PXD023772

PXD023772 is an original dataset announced via ProteomeXchange.

Title	Transcriptome and proteome analysis of the human corneal endothelium and aqueous humor reveal endothelial cell dysfunction markers
Description	Background: The underlying mechanism as well as diagnostic molecular biomarkers for corneal endothelial cell dysfunction (CECD) remain elusive. This study aimed to elucidate molecular mechanism of CECD via proteomic and transcriptomic approaches and identify novel biomarkers in aqueous humor (AH) directly associated with changes in CECs. Methods: We carried out an in-depth proteomic analysis of patient-derived AH as well as transcriptomic analysis of CECs from the same patients with bullous keratopathy (BK) resulting from CECD. Selected candidate proteins were further verified by individual data-independent acquisition and immunoassay using further AH samples from CECD patients. Findings: Using integrated multi-omics analysis, systematic alterations of proteins in AH and mRNA in CECs were mainly associated with the immune mechanism during BK development. Through individual proteomic verification via DIA analysis, three AH proteins were determined as potential predictive markers including TIMP1, FCGBP, and ANGPTL7. Finally, we confirmed these biomarkers using immunoassay in individual AH samples of CECD. Interpretation: Our multi-omics analysis may provide valuable insights into the disease-specific process as well as possibility of biofluid biomarkers in CECD. Further investigation with large scale is required to determine whether AH biomarkers are useful as liquid biopsy targets for the assessment of CEC function
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:09:19.879.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jae Hun Jung
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-24 23:29:10	ID requested
⏵ 1	2024-01-26 06:09:20	announced

10.3390/ijms242015354;

Moon CE, Kim CH, Jung JH, Cho YJ, Choi KY, Han K, Seo KY, Lee HK, Ji YW, Integrated Analysis of Transcriptome and Proteome of the Human Cornea and Aqueous Humor Reveal Novel Biomarkers for Corneal Endothelial Cell Dysfunction. Int J Mol Sci, 24(20):(2023) [pubmed]

submitter keyword: proteomics, aqueous humor,Corneal endothelial cell dysfunction, transcriptomics, biomarker, bullous keratopathy

Jae Hun Jung
contact affiliation	Yonsei university
contact email	jaehun.jung.88@gmail.com
lab head
Jae Hun Jung
contact affiliation	KHU
contact email	ultramanyo@naver.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023772
     1. Label: PRIDE project
     2. Name: Transcriptome and proteome analysis of the human corneal endothelium and aqueous humor reveal endothelial cell dysfunction markers