PXD023734

PXD023734 is an original dataset announced via ProteomeXchange.

Title	Label-free proteomic analysis of neuroglobin-knockout breast cancer cells
Description	Neuroglobin (NGB) is an oxygen-binding protein that takes part in several processes that safeguard redox homeostasis, gaining an important protective role in neuronal and extra-neuronal cells. NGB remarkably exerts its function upon up-regulation by NGB-inducible molecules such as 17β-estradiol (E2) and H2O2. Despite this, the essential mechanisms related to NGB function remain still undefined. Human MCF-7 breast cancer cells knocked out (KO) for NGB gene obtained using CRISPR/Cas9 technology were analyzed by shotgun label-free quantitative proteomics in comparison with control cells. Differential proteomics experiments were also performed using NGB-KO cells after treatment with E2, H2O2, and E2+H2O2 versus their corresponding non-treated controls.
HostingRepository	PRIDE
AnnounceDate	2021-07-16
AnnouncementXML	Submission_2021-07-15_22:40:11.689.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Costanzo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-21 03:54:34	ID requested
⏵ 1	2021-07-15 22:40:12	announced

Costanzo M, Fiocchetti M, Ascenzi P, Marino M, Caterino M, Ruoppolo M, Proteomic and Bioinformatic Investigation of Altered Pathways in Neuroglobin-Deficient Breast Cancer Cells. Molecules, 26(8):(2021) [pubmed]

submitter keyword: Neuroglobin, NGB, label-free proteomics, S-Trap

Michele Costanzo
contact affiliation	Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II", Italy
contact email	michele.costanzo@unina.it
lab head
Michele Costanzo
contact affiliation	Department of Molecular Medicine and Medical Biotechnology - University of Naples Federico II
contact email	michele.costanzo@unina.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023734
     1. Label: PRIDE project
     2. Name: Label-free proteomic analysis of neuroglobin-knockout breast cancer cells