PXD023721

PXD023721 is an original dataset announced via ProteomeXchange.

Title	Cathepsin F and Fibulin-1 as novel diagnostic biomarkers for Brain Metastasis of Non-Small Cell Lung Cancer
Description	Background: There is a paucity of non-invasive markers for early detection of brain metastasis (BM) in patients with non-small cell lung cancer (NSCLC). Secretory proteomic approach based on quantitative tandem mass tag is believed to offer bright prospects for screening potential serum biomarkers for diseases. Methods: We used the secretory proteomics to identify up-regulated proteins in the highly brain metastatic cell line compared to the parental lung cancer cell line. Enzyme linked immunoassay and immunohistochemistry were performed on non-small cell lung cancer patients with brain metastasis and control samples from each cohort to verify the expression levels of candidate proteins. A logistic regression model was used to develop a biomarker panel composed of Cathepsin F (CTSF) and Fibulin-1 (FBLN1), and then an independent validation data set was used for verification. The diagnostic accuracy was evaluated by receiver operating characteristic (ROC) curve analysis. Changes in serum CTSF levels of patients were tracked to monitor the effect of treatment, and progression-free survival (PFS) and overall survival (OS) were analyzed to assess their prognostic relevance. Results: CTSF and FBLN1 levels were specifically upregulated in sera and tissues of patients with NSCLC BM. Importantly, the combined diagnostic performance of CTSF and FBLN1 was superior to their individual diagnostic performance (sensitivity and specificity). CTSF serum changes were found to reflect the therapeutic response of patients with NSCLC BM and the trends of resistance and progression were detected earlier than the MRI changes. Elevated expression of CTSF in NSCLC BM tissues was associated with poor PFS. Moreover, CTSF tissue expression was found to be an independent prognostic factor. Conclusions: CTSF and FBLN1 are potential novel and specific serum markers for early diagnosis of BM in patients with NSCLC. CTSF can also be used as a biomarker for monitoring therapeutic efficacy and prognostic assessment.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:46:29.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-21 00:06:58	ID requested
⏵ 1	2022-08-11 23:46:31	announced

Wei S, Liu W, Xu M, Qin H, Liu C, Zhang R, Zhou S, Li E, Liu Z, Wang Q, Cathepsin F and Fibulin-1 as novel diagnostic biomarkers for brain metastasis of non-small cell lung cancer. Br J Cancer, 126(12):1795-1805(2022) [pubmed]

submitter keyword: NSCLC brain metastasis

proteomics

Diagnosis

Follow-up

Prognosis.

Qi Wang
contact affiliation	The Second Hospital of Dalian Medical University
contact email	wqdlmu@163.com
lab head
Qi Wang
contact affiliation	The Second Hospital of Dalian Medical University
contact email	wqdlmu@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023721
     1. Label: PRIDE project
     2. Name: Cathepsin F and Fibulin-1 as novel diagnostic biomarkers for Brain Metastasis of Non-Small Cell Lung Cancer