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PXD023708

PXD023708 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics profiling of human synovial fluid suggests global increased protein interplay in early-osteoarthritis (OA) and lost in late-stage OA
DescriptionThe underlying molecular mechanisms in osteoarthritis (OA) development are largely unknown. This study explores the proteome and the pairwise interplay of proteins on a global level in synovial fluid from patients with late-stage knee OA (arthroplasty), early knee OA (arthroscopy due to degenerative meniscal tear) and from deceased controls without knee OA. Synovial fluid samples were analyzed using state-of-the-art mass spectrometry with data-independent acquisition. The differential expression of the proteins detected was clustered and evaluated with data mining strategies and a multilevel model. Group-specific slopes of associations were estimated between expressions of each pair of identified proteins to assess the co-expression (i.e. interplay) between the proteins in each group. More proteins were increased in early-OA vs controls than late-stage OA vs controls. For most of these proteins, the fold changes between late-stage OA vs controls and early stage OA vs controls were remarkably similar suggesting potential involvement in the OA process. Further, for the first time this study illustrated distinct patterns in protein co-expression suggesting that the global interplay between the protein machinery is increased in early-OA and lost in late-stage OA. Further efforts should probably focus on earlier stages of the disease than previously considered.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:31:00.945.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNeserin Ali
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-20 08:14:46ID requested
12022-01-27 10:13:07announced
22022-01-30 13:32:30announced2022-01-30: Updated publication reference for PubMed record(s): 35074580.
32024-10-22 05:31:01announced2024-10-22: Updated project metadata.
Publication List
Ali N, Turkiewicz A, Hughes V, Folkesson E, Tj, ö, rnstand J, Neuman P, Ö, nnerfjord P, Englund M, Proteomics Profiling of Human Synovial Fluid Suggests Increased Protein Interplay in Early-Osteoarthritis (OA) That Is Lost in Late-Stage OA. Mol Cell Proteomics, 21(3):100200(2022) [pubmed]
10.1016/j.mcpro.2022.100200;
Keyword List
submitter keyword: DIA, Synovial fluid,Osteoarthritis
Contact List
Neserin Ali
contact affiliationLund University
contact emailneserin.ali@med.lu.se
lab head
Neserin Ali
contact affiliationLund University
contact emailneserin.ali@med.lu.se
dataset submitter
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Dataset FTP location
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