PXD023680

PXD023680 is an original dataset announced via ProteomeXchange.

Title	Loss of Cep70 function affects acrosome biogenesis and flagella formation during spermiogenesis
Description	The spermatogenesis process is complex and delicate, and any error in any step may cause spermatogenesis arrested and even male infertility. Through mutation screening of patients with clinical azoospermia, we found a heterozygous site of the same mutation on CEP70. The centrosome protein 70 (Cep70) is involved in the regulation of microtubule assembly and shows a high expression trend during human spermatogenesis, but the specific mechanism of this protein in spermatogenesis is still unknown. Therefore, we deleted Cep70 in mice and founded that the deletion of Cep70 causes abnormal spermatogenesis and leads to male sterility. We found that knockout of Cep70 did not affect the prophase of meiosis I, but led to higher levels of thyroid hormone secretion, male germ cell apoptosis and abnormal spermiogenesis. By TEM and SEM analysis, we found that deletion of Cep70 results in abnormal formation of flagella and acrosome during spermiogenesis. TMT-labeled quantitative proteomic analysis revealed that the absence of Cep70 led to the significantly decrease of proteins associated with the formation of the flagella, head and acrosome of sperm and microtubule cytoskeleton according to the proteomic data. Taken together, our results show that Cep70 is essential for the acrosome biogenesis and flagella formation during spermiogenesis.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-09_23:43:43.410.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qiang Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-19 22:20:26	ID requested
⏵ 1	2021-09-09 23:43:44	announced

Liu Q, Guo Q, Guo W, Song S, Wang N, Chen X, Sun A, Yan L, Qiao J, Loss of CEP70 function affects acrosome biogenesis and flagella formation during spermiogenesis. Cell Death Dis, 12(5):478(2021) [pubmed]

submitter keyword: Cep70

male infertility

acrosome biogenesis

flagella formation

spermiogenesis

Qiang Liu
contact affiliation	Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China
contact email	liuqiangtaian2008@163.com
lab head
Qiang Liu
contact affiliation	Peking University Third Hospital
contact email	liuqiangtaian2008@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023680
     1. Label: PRIDE project
     2. Name: Loss of Cep70 function affects acrosome biogenesis and flagella formation during spermiogenesis