PXD023679

PXD023679 is an original dataset announced via ProteomeXchange.

Title	Delineation of the pH-responsive regulon controlled by the Helicobacter pylori ArsRS two2 component system
Description	Helicobacter pylori encounters a wide range of pH within the human stomach. In a comparison of H. pylori cultured in vitro under neutral or acidic conditions, about 15% of genes are26 differentially expressed, and corresponding changes are detectable for many of the encoded proteins. The ArsRS two-component system (TCS), comprised of the sensor kinase ArsS and its cognate response regulator ArsR, has an important role in mediating pH-responsive changes in H. pylori gene expression. In this study, we sought to delineate the pH-responsive ArsRS regulon and further define the role of ArsR in pH-responsive gene expression. We compared H. pylori strains containing an intact ArsRS system with an arsS null mutant or strains containing site32 specific mutations of a conserved aspartate residue (D52) in ArsR, which is phosphorylated in response to signals relayed by the cognate sensor kinase ArsS. We identified 178 genes that were pH-responsive in strains containing an intact ArsRS system but not in ∆arsS or arsR mutants. These constituents of the pH-responsive ArsRS regulon include genes involved in acid acclimatization (ureAB, amidases), oxidative stress responses (katA, sodB), transcriptional regulation related to iron or nickel homeostasis (fur, nikR), and genes encoding outer membrane proteins [including sabA, alpA, alpB, hopD (labA), and horA]. When comparing H. pylori strains containing an intact ArsRS TCS with arsRS mutants, each cultured at neutral pH, relatively few genes are differentially expressed. Collectively, these data suggest that ArsRS41 mediated gene regulation has an important role in H. pylori adaptation to changing pH conditions.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_11:27:28.405.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hayes McDonald
SpeciesList	scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-19 22:10:32	ID requested
⏵ 1	2022-02-17 11:27:29	announced

Loh JT, Shum MV, Jossart SDR, Campbell AM, Sawhney N, McDonald WH, Scholz MB, McClain MS, Forsyth MH, Cover TL, Delineation of the pH-Responsive Regulon Controlled by the Helicobacter pylori ArsRS Two-Component System. Infect Immun, 89(4):(2021) [pubmed]

submitter keyword: gene regulation, two-component signal transduction systems, RNA-seq

Timothy L. Cover
contact affiliation	Department of Medicine, Department of Pathology, Microbiology and Immunology Vanderbilt University School of Medicine; Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN
contact email	timothy.l.cover@vumc.org
lab head
Hayes McDonald
contact affiliation	Vanderbilt University
contact email	hayes.mcdonald@vanderbilt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023679
     1. Label: PRIDE project
     2. Name: Delineation of the pH-responsive regulon controlled by the Helicobacter pylori ArsRS two2 component system