PXD023678

PXD023678 is an original dataset announced via ProteomeXchange.

Title	Major determinants of airway epithelial cell sensitivity to S. aureus alpha-toxin: Disposition of toxin heptamers by extracellular vesicle formation and lysosomal degradation
Description	Alpha-toxin is a major virulence factor of Staphylococcus aureus and plays an important role in S. aureus-induced pneumonia. Different sensitivities toward the toxin of various host cell types have been observed. In this study we investigated internalization of pore-containing areas of the plasma membrane in these cell types as well as potential pathways for heptamer degradation (lysosomal, proteasomal) or disposal (formation of exosomes/microvesicles containing toxin pores). Comparisons of heptamer degradation rates under inhibition of lysosomal or proteasomal degradation revealed that an important route of heptamer degradation at least in S9 cells seemed to be the lysosomal pathway while proteasomal degradation did not seem to be relevant. Exosomes prepared from culture supernatants of toxin-exposed S9 cells, contained alpha-toxin as well as low amounts of marker proteins of exosomes and microvesicles. These results indicate that the ability of lysosomal degradation of internalized toxin heptamers may be the most important determinant of toxin-resistance of some types of airway epithelial cells. In this study, we compared the proteomes of rHla treated (90 min), 8 h starvation and, 48 h starvation for the S9 cells by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:21:19.791.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	christian hentschker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-19 22:10:07	ID requested
1	2021-04-20 02:05:53	announced
⏵ 2	2024-10-22 05:21:25	announced	2024-10-22: Updated project metadata.

10.3390/toxins13030173;

M, ö, ller N, Ziesemer S, Hentschker C, V, ö, lker U, Hildebrandt JP, Alpha-Toxin: Disposal of Toxin Heptamers by Extracellular Vesicle Formation and Lysosomal Degradation. Toxins (Basel), 13(3):(2021) [pubmed]

submitter keyword: cellular sensitivity, alpha-toxin, airway epithelial cells, virulence factors,Staphylococcus aureus, transmembrane pores

Jan-Peter Hildebrandt
contact affiliation	University of Greifswald, Animal Physiology and Biochemistry, Felix Hausdorff-Strasse 1, D - 17489 Greifswald, Germany
contact email	jph@uni-greifswald.de
lab head
christian hentschker
contact affiliation	uni
contact email	christianhentschker@web.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD023678

PRIDE project URI

• PRIDE
  1. PXD023678
     1. Label: PRIDE project
     2. Name: Major determinants of airway epithelial cell sensitivity to S. aureus alpha-toxin: Disposition of toxin heptamers by extracellular vesicle formation and lysosomal degradation