PXD023664

PXD023664 is an original dataset announced via ProteomeXchange.

Title	Identification of a TOR-regulated phosphoproteome and the regulation of cellular protein synthesis
Description	We describe an in depth analysis in fission yeast of changes in the phosphoproteome after inhibition of the TOR protein kinases. A total of 756 phosphosites on 316 proteins showed significant and rapid changes in phosphorylation levels after TOR kinase inhibition when the rate of protein synthesis reduced by 90%. These proteins were involved in a range of cellular processes including 4 that featured strongly: signalling-related processes, translation, cell polarity and actin cytoskeletal organisation, and vesicle-mediated transport and autophagy. We propose that these processes have major roles in TOR regulated cellular growth control. We also show that the S6 protein kinases have no role in immediate TOR regulated cellular protein synthesis, but in contrast there is a role for Tif471, the translation initiation factor homologue of eIF4G. This protein becomes rapidly phosphorylated after TORC1 inhibition in particular, but if that phosphorylation is prevented, as demonstrated by mutations on the specific phosphosites, the inhibition of protein synthesis rate is reduced. We propose that this factor is a downstream target of the TOR pathway, with a role in partially regulating global cellular protein synthesis.
HostingRepository	PRIDE
AnnounceDate	2021-07-09
AnnouncementXML	Submission_2021-07-09_03:06:41.783.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Jones
SpeciesList	scientific name: Schizosaccharomyces pombe 972h-; NCBI TaxID: 284812;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-19 04:41:19	ID requested
⏵ 1	2021-07-09 03:06:42	announced

Dataset with its publication pending

submitter keyword: S. pombe, fission yeast, TOR, TMT, Phosphoproteomics

Paul Nurse
contact affiliation	The Francis Crick Institute
contact email	Paul.Nurse@crick.ac.uk
lab head
Andrew Jones
contact affiliation	The Francis Crick Institute
contact email	Andrew.Jones@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023664
     1. Label: PRIDE project
     2. Name: Identification of a TOR-regulated phosphoproteome and the regulation of cellular protein synthesis