PXD023661
PXD023661 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic evidence for the silk fibroin genes of spider mites (order Trombidiformes: family Tetranychidae) |
| Description | Spider mites are a group of arachnids belonging in the family Tetranychidae, known to produce nano-scale silk fibers characterized by high Young’s modulus. Silk fibroin gene of spider mites has been computationally suggested through genomic analysis of Tetranychus urticae, but it is yet to be confirmed by proteomic evidences. In this work, we sequenced and assembled the transcriptome from two genera of spider mites, Tetranychus kanzawai and Panonychus citri, and combined with the silk proteomics of T. urticae and P. citri to characterize the fibroin genes through comparative genomics and multi-omics analysis. As a result, two fibroins were identified, that are different genes from the previous computational prediction. Amino acid composition and secondary structure suggests similarity to aciniform or cylindrical spidroins of spider silk, which partly mirrors their mechanical property exhibiting high Young’s modulus. Availability of full-length fibroin sequences of spider mites facilitates the study of the evolution of silk genes that emerged in multiple lineages sometimes in convergent manner, and in the industrial application of artificial protein fibers through the study of the amino acid sequence and the resulting mechanical properties of these silks. |
| HostingRepository | jPOST |
| AnnounceDate | 2021-03-24 |
| AnnouncementXML | Submission_2022-09-18_02:51:30.113.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD023661 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Kazuharu Arakawa |
| SpeciesList | scientific name: Tetranychus kanzawai; NCBI TaxID: 50028; scientific name: Tetranychus urticae; NCBI TaxID: 32264; scientific name: Panonychus citri; NCBI TaxID: 50023; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-01-18 21:11:01 | ID requested | |
| 1 | 2021-03-23 23:51:49 | announced | |
| 2 | 2021-03-25 05:25:42 | announced | 2021-03-25: Updated PubMed. |
| ⏵ 3 | 2022-09-18 02:51:30 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Arakawa K, Mori M, Kono N, Suzuki T, Gotoh T, Shimano S, Proteomic evidence for the silk fibroin genes of spider mites (Order Trombidiformes: Family Tetranychidae). J Proteomics, 239():104195(2021) [pubmed] |
Keyword List
| submitter keyword: acari, spider mites, silk, fibroin |
Contact List
| Kazuharu Arakawa | |
|---|---|
| lab head | |
| Kazuharu Arakawa | |
| contact affiliation | Institute for Advanced Biosciences, Keio University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001061/ |
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