PXD023660

PXD023660 is an original dataset announced via ProteomeXchange.

Title	The injury environment critically influences the brain-wide input connectome of transplanted neurons
Description	Cell transplantation is a promising approach for reconstruction of neuronal circuits after brain damage. Transplanted neurons integrate with remarkable specificity into circuitries of the mouse cerebral cortex affected by neuronal ablation. However, it remains unclear how neurons perform in a local environment undergoing reactive gliosis, inflammation, macrophage infiltration and scar formation, as in brain trauma. To elucidate this, we transplanted cells from the embryonic murine cerebral cortex into stab-injured, inflamed-only, or intact cortex of adult mice. Brain-wide quantitative connectomics unraveled graft inputs from correct regions across the brain in all conditions, with pronounced quantitative differences: scarce in intact or inflamed brain, versus exuberant after trauma. In the latter, excessive synapse pruning follows the initial overshoot of connectivity resulting in only a few input connections left. Proteomic profiling identifies candidate molecules involved in the synaptic yield, a pivotal parameter to tailor for functional restoration of neuronal circuits.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_02:31:37.537.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023660
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stefanie Hauck
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-18 07:21:21	ID requested
⏵ 1	2022-08-12 02:31:38	announced

Grade S, Thomas J, Zarb Y, Thorwirth M, Conzelmann KK, Hauck SM, G, ö, tz M, Brain injury environment critically influences the connectivity of transplanted neurons. Sci Adv, 8(23):eabg9445(2022) [pubmed]

submitter keyword: Transplantation, Neuronal replacement, Circuit mapping, Synaptic yield, Inflammation, Injury proteome, Cell therapy

Stefanie M. Hauck
contact affiliation	Research Unit Protein Science, Helmholtz Zentrum München
contact email	hauck@helmholtz-muenchen.de
lab head
Stefanie Hauck
contact affiliation	Research Unit Protein Science, Helmholtz Zentrum München
contact email	hauck@helmholtz-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023660
     1. Label: PRIDE project
     2. Name: The injury environment critically influences the brain-wide input connectome of transplanted neurons