PXD023655

PXD023655 is an original dataset announced via ProteomeXchange.

Title	Determining the hydroxylation status of NAA10-Trp38
Description	NAA10 is the major human N-terminal acetyltransferase (NAT). The KAT activity of NAA10 towards hypoxia-inducible factor 1α (HIF-1α) was recently reported to depend on the hydroxylation at Trp38 of NAA10 by factor inhibiting HIF-1α (FIH). As a consequence, Trp38 hydroxylation status was proposed to act as a general NAA10-switch between its NAT and KAT state. We attempted to quantify the degree of hydroxylation of NAA10. We could not detect any hydroxylation of Trp38 of NAA10 in several human cell lines.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_13:32:54.170.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rasmus Ree
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-18 02:25:29	ID requested
⏵ 1	2022-02-16 13:32:54	announced

Ree R, Krogstad K, McTiernan N, Jakobsson ME, Arnesen T, Hydroxylation of the Acetyltransferase NAA10 Trp38 Is Not an Enzyme-Switch in Human Cells. Int J Mol Sci, 22(21):(2021) [pubmed]

submitter keyword: NAA10, NAT, hydroxylation, Asp-OH, Asn-OH, Trp-OH

Thomas Arnesen
contact affiliation	Department of Biomedicine, University of Bergen
contact email	thomas.arnesen@uib.no
lab head
Rasmus Ree
contact affiliation	University of Bergen
contact email	rasmus.ree@uib.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023655
     1. Label: PRIDE project
     2. Name: Determining the hydroxylation status of NAA10-Trp38