PXD023650

PXD023650 is an original dataset announced via ProteomeXchange.

Title	Robust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting
Description	Micro-flow liquid chromatography tandem mass spectrometry (µLC–MS/MS) is increasingly becoming an alternative to nano-LC–MS/MS for the analysis of proteomes. We have recently demonstrated the potential of a µLC–MS/MS system operating with a 1 mm i.d. × 150 mm column and at a flow rate of 50 µl/min for high-throughput applications. Based on the analysis of ~38,000 samples measured on two instruments over the past two years, we show that the approach is extremely robust. Up to 1,500 analysis were performed within one month and >14,000 samples could be analyzed on a single column without loss of chromatographic performance. Samples included proteomes of cell lines, animal tissue and human body fluids, which were analyzed either with or without prior peptide fractionation or stable isotope labeling. We show that the µLC–MS/MS system is capable of measuring ~2,700 proteins from human plasma and ~5,200 proteins from human urine within one day, demonstrating its potential for in-depth as well as high-throughput clinical application.
HostingRepository	PRIDE
AnnounceDate	2021-02-22
AnnouncementXML	Submission_2021-02-21_23:22:11.831.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yangyang Bian
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-18 00:09:23	ID requested
⏵ 1	2021-02-21 23:22:12	announced

Bian Y, Bayer FP, Chang YC, Meng C, Hoefer S, Deng N, Zheng R, Boychenko O, Kuster B, 000 Runs and Counting. Anal Chem, 93(8):3686-3690(2021) [pubmed]

submitter keyword: Micro-flow LC, Proteomics, Urine, Plasma, 1.0 mm ID column

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technische Universitaet Muenchen
contact email	kuster@tum.de
lab head
Yangyang Bian
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich
contact email	yangyang.bian@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD023650

PRIDE project URI

• PRIDE
  1. PXD023650
     1. Label: PRIDE project
     2. Name: Robust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting