PXD023642

PXD023642 is an original dataset announced via ProteomeXchange.

Title	human adenovirus-host protein interactions
Description	As intracellular parasites, viruses exploit cellular proteins at every stage of infection. Adenovirus related outbreaks cause severe acute respiratory illnesses and conjunctivitis, with no specific antiviral therapy yet available. Herein, we investigate human adenovirus (HAdV-D37) pIIIa-host protein interactions by affinity purification and mass spectrometry (AP-MS) screens. We demonstrate that viral pIIIa interacts with ubiquitin-specific protease 9x (USP9x) and Ran-binding protein 2 (RANBP2). As intracellular parasites, viruses exploit cellular proteins at every stage of infection. Adenovirus related outbreaks cause severe acute respiratory illnesses and conjunctivitis, with no specific antiviral therapy yet available. Herein, we investigate human adenovirus (HAdV-D37) pIIIa-host protein interactions by affinity purification and mass spectrometry (AP-MS) screens. We demonstrate that viral pIIIa interacts with ubiquitin-specific protease 9x (USP9x) and Ran-binding protein 2 (RANBP2).
HostingRepository	PRIDE
AnnounceDate	2022-05-30
AnnouncementXML	Submission_2022-05-30_08:56:36.302.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohamed Ismail Ashrafali
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-17 23:46:56	ID requested
⏵ 1	2022-05-30 08:56:36	announced

Dataset with its publication pending

submitter keyword: adenovirus, LC-MS/MS, pIIIa, affinity purification, casid protein

James Chodosh
contact affiliation	Professor of Ophthalmology Massachusetts Eye and Ear Harvard Medical School 243 Charles Street Boston, MA 02148
contact email	James_Chodosh@meei.harvard.edu
lab head
Mohamed Ismail Ashrafali
contact affiliation	Massachusetts Eye and Ear, Harvard Medical School
contact email	ismailcmc@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD023642

PRIDE project URI

• PRIDE
  1. PXD023642
     1. Label: PRIDE project
     2. Name: human adenovirus-host protein interactions