PXD023623

PXD023623 is an original dataset announced via ProteomeXchange.

Title	PIM1 phosphorylation of 14-3-3 ζ in vitro
Description	We previously identified 14-3-3 ζ S64 as a substrate of PIM1 in an in vivo chemical genetic screen. To verify this phosphorylation event and determine whether S64 is the predominant phosphorylation site, we performed in vitro phosphorylation of 14-3-3 ζ by PIM1.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_07:44:06.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikita Vasilyev
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-15 09:05:40	ID requested
⏵ 1	2021-11-25 07:44:07	announced

Dataset with its publication pending

submitter keyword: PIM1, 14-3-3 ζ, protein phosphorylation, kinase

Evgeny Nudler
contact affiliation	Julie Wilson Anderson Professor of Biochemistry, Department of Biochemistry and Molecular Pharmacology, NYU Grossman School of Medicine, US
contact email	evgeny.nudler@nyulangone.org
lab head
Nikita Vasilyev
contact affiliation	NYU School of Medicine
contact email	nikita.vasilyev@nyulangone.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD023623

PRIDE project URI

• PRIDE
  1. PXD023623
     1. Label: PRIDE project
     2. Name: PIM1 phosphorylation of 14-3-3 ζ in vitro