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PXD023613

PXD023613 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMinute-scale proteomics with Scanning SWATH and high-flow chromatography
DescriptionGreat advances have been made to quantify proteomes at depth and high precision. However, the inherent complexity of the proteome still creates huge challenges to deal with large sample series and longitudinal experiments. Here we report a data-independent acquisition (DIA) method, Scanning SWATH, and new software algorithms, that combine the advantages of data-dependent and DIA acquisition. Exploiting a continuous movement of the precursor isolation window to assign precursor mass to the MS2 fragment traces, Scanning SWATH increases the precursor identifications of up to 70% compared to conventional DIA methods on 1-5 minute chromatographic gradients. Moreover, we achieve significant acceleration of the mass spectrometric duty cycles, facilitating the generation of quantitative precise proteomes with high-flow (800 µL/min) chromatography and gradients that can be as fast as 30 seconds. We demonstrate the application of ultra-fast proteomics in drug mode-of-action screening. Scanning SWATH proteomes capture the mode-of-action of azoles and statins acting as fungistatic drugs.
HostingRepositoryPRIDE
AnnounceDate2021-03-02
AnnouncementXMLSubmission_2021-03-29_02:53:26.234.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristoph Messner
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-15 06:24:23ID requested
12021-03-02 07:54:21announced
22021-03-29 02:53:27announced2021-03-29: Updated publication reference for PubMed record(s): 33767396.
Publication List
Messner CB, Demichev V, Bloomfield N, Yu JSL, White M, Kreidl M, Egger AS, Freiwald A, Ivosev G, Wasim F, Zelezniak A, Jürgens L, Suttorp N, Sander LE, Kurth F, Lilley KS, Mülleder M, Tate S, Ralser M, Ultra-fast proteomics with Scanning SWATH. Nat Biotechnol, ():(2021) [pubmed]
Keyword List
submitter keyword: High-throughput, Scanning SWATH, data-independent acquisition, high-flow chromatography, drug screen
Contact List
Markus Ralser
contact affiliationCharité Universitätsmedizin Berlin, Department of Biochemistry, Berlin, Germany
contact emailmarkus.ralser@charite.de
lab head
Christoph Messner
contact affiliationFrancis Crick Institute
contact emailchristoph.messner@crick.ac.uk
dataset submitter
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