PXD023604

PXD023604 is an original dataset announced via ProteomeXchange.

Title	Yeast mitochondrial succinylome: Implications for mitochondrial DNA maintenance
Description	Together with other post-translational modifications, acylation of proteins is a powerful means of regulation of their activity. Some acylation types occur nonenzymatically and are driven by an increase in the concentration of acyl group donors. Lysine succinylation has a profound effect on the corresponding site within the protein as it changes the charge of the residue. In eukaryotes it predominantly affects mitochondrial proteins because the donor of succinate, succinyl-coenzyme A, is primarily generated in the tricarboxylic acid (TCA) cycle. Although numerous succinylated mitochondrial proteins were identified in Saccharomyces cerevisiae, a detailed characterization of yeast mitochondrial succinylome is lacking. Here we performed a proteomic mass spectrometry analysis of purified yeast mitochondria and detected 313 succinylated mitochondrial proteins with 1762 novel succinylation sites.
HostingRepository	PRIDE
AnnounceDate	2021-10-26
AnnouncementXML	Submission_2021-10-26_06:53:20.727.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barath
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	N6-succinyl-L-lysine; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-14 09:03:16	ID requested
⏵ 1	2021-10-26 06:53:21	announced

Frankovsky J, Keresztesov, á B, Bellov, á J, Kunov, á N, Č, anigov, á N, Hanakova K, Bauer JA, Ondrovi, č, ov, á G, Luk, á, č, ov, á V, Siv, á, kov, á B, Zdrahal Z, Pevala V, Proch, á, zkov, á K, Nosek J, Bar, á, th P, Kutejova E, Tomaska L, The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids. J Biol Chem, 297(4):101155(2021) [pubmed]

submitter keyword: yeast, mitochondria, PTM, succinylation

Peter Barath
contact affiliation	Department of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
contact email	peter.barath@savba.sk
lab head
Peter Barath
contact affiliation	Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023604
     1. Label: PRIDE project
     2. Name: Yeast mitochondrial succinylome: Implications for mitochondrial DNA maintenance