PXD023595

PXD023595 is an original dataset announced via ProteomeXchange.

Title	A multi-OMICs approach sheds light on the higher yield phenotype and enhanced abiotic stress tolerance in tobacco lines expressing the carrot lycopene β-cyclase1 gene
Description	Here, we combine OMICs (RNAseq, proteomics, and metabolomics) approaches to advance our understanding of the broader effect of LCYB expression on the tobacco genome and metabolism. DcLCYB1 gene with accelerated development, increased carotenoid content, photosynthetic efficiency, and yield might be of general interest in crop species as a strategy to accelerate development and increase biomass production under field conditions. Upon DcLCYB1 expression, the tobacco genome (~2000 genes), proteome (~700 proteins), and metabolome (26 metabolites) showed a high number of changes in the genes involved in metabolic processes related to cell wall, lipids,glycolysis, and secondary metabolism. Gene and protein networks revealed clusters of interacting genes and proteins mainly involved in ribosome and RNA metabolism and translation. In addition, abiotic stress-related genes and proteins were mainly upregulated in the transgenic lines. This was well in line with an enhanced stress (high light, salt, and H2O2) tolerance response in all the transgenic lines compared with the wild type. Altogether, our results show an extended and coordinated response beyond the chloroplast (nucleus and cytosol) at the genome, proteome, and metabolome levels, supporting enhanced plant growth under normal and stress conditions. This final evidence completes the set of benefits conferred by the expression of the DcLCYB1 gene, making it a very promising bioengineering tool to generate super crops.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-09_16:19:28.520.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ewelina Sokolowska
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-14 04:38:06	ID requested
⏵ 1	2021-09-09 16:19:29	announced

Moreno JC, Martinez-Jaime S, Kosmacz M, Sokolowska EM, Schulz P, Fischer A, Luzarowska U, Havaux M, Skirycz A, Gene. Front Plant Sci, 12():624365(2021) [pubmed]

submitter keyword: β-carotene, carotenoids, high light and salt stress, lycopene β-cyclase, OMICs, ROS, Nicotiana tabacum cv Xanthi, transcription factors

Aleksandra Skirycz
contact affiliation	1Max Planck Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg1 D-14476, Potsdam-Golm, Germany.
contact email	skirycz@mpimp-golm.mpg.de
lab head
Ewelina Sokolowska
contact affiliation	Max Planck Institute of Molecular Plant Physiology Wissenschaftspark Am Mühlenberg 1 14476 Potsdam-Golm
contact email	esokolowska@mpimp-golm.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023595

PRIDE project URI

• PRIDE
  1. PXD023595
     1. Label: PRIDE project
     2. Name: A multi-OMICs approach sheds light on the higher yield phenotype and enhanced abiotic stress tolerance in tobacco lines expressing the carrot lycopene β-cyclase1 gene