PXD023593

PXD023593 is an original dataset announced via ProteomeXchange.

Title	Structural analysis of neurofibromin by crosslinking mass spectrometry
Description	Neurofibromin (NF1) is a fundamental inhibitor of cell growth that is conserved from yeast to humans. NF1 negatively regulates oncogenic RAS proteins by accelerating the hydrolysis of RAS-bound GTP. This activity blocks growth factor signalling upstream of both mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. However, the structure and regulatory mechanisms of NF1 have remained elusive. Here we report crosslinking mass spectrometry analysis of the 320 kDa NF1 protein.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:56.894.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ralf Schittenhelm
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-14 03:31:55	ID requested
1	2022-08-12 06:13:07	announced
⏵ 2	2023-11-14 08:51:57	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: cancer,Neurofibromin, tumour-suppressor protein

Andrew Ellisdon
contact affiliation	Biomedicine Discovery Institute, Monash University, Clayton 3800, Victoria, Australia
contact email	andrew.ellisdon@monash.edu
lab head
Ralf Schittenhelm
contact affiliation	Monash University
contact email	ralf.schittenhelm@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD023593

PRIDE project URI

• PRIDE
  1. PXD023593
     1. Label: PRIDE project
     2. Name: Structural analysis of neurofibromin by crosslinking mass spectrometry