PXD023586 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | AP-MS of helicase Upf1 in PC12 cells |
Description | DNA/RNA helicase Upf1 seems to interact with axonal transcripts in response to nerve growth factor (NGF, A. Ludanyi, M. Gaspari and A. Riccio, unpublished data). In order to shed light on Upf1 mechanism of action and to identify potential associations with molecules having cleavage activity, we performed an AP-MS experiment comprising Upf1 immunoprecipitation, on-beads digestion, isotopic labelling (18O) and quantitative LC-MS/MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-10 |
AnnouncementXML | Submission_2021-09-09_16:12:21.670.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marco Gaspari |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | (18)O label at both C-terminal oxygens |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-14 01:49:35 | ID requested | |
⏵ 1 | 2021-09-09 16:12:22 | announced | |
Publication List
Andreassi C, Luisier R, Crerar H, Darsinou M, Blokzijl-Franke S, Lenn T, Luscombe NM, Cuda G, Gaspari M, Saiardi A, Riccio A, Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity. Cell Rep, 34(8):108778(2021) [pubmed] |
Keyword List
submitter keyword: Protein complex, IMPA1, HuD, Ago2 |
Contact List
Marco Gaspari |
contact affiliation | Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, Italy |
contact email | gaspari@unicz.it |
lab head | |
Marco Gaspari |
contact affiliation | University of Catanzaro |
contact email | gaspari@unicz.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023586 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023586
- Label: PRIDE project
- Name: AP-MS of helicase Upf1 in PC12 cells