PXD023586

PXD023586 is an original dataset announced via ProteomeXchange.

Title	AP-MS of helicase Upf1 in PC12 cells
Description	DNA/RNA helicase Upf1 seems to interact with axonal transcripts in response to nerve growth factor (NGF, A. Ludanyi, M. Gaspari and A. Riccio, unpublished data). In order to shed light on Upf1 mechanism of action and to identify potential associations with molecules having cleavage activity, we performed an AP-MS experiment comprising Upf1 immunoprecipitation, on-beads digestion, isotopic labelling (18O) and quantitative LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-09_16:12:21.670.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marco Gaspari
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	(18)O label at both C-terminal oxygens
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-14 01:49:35	ID requested
⏵ 1	2021-09-09 16:12:22	announced

Andreassi C, Luisier R, Crerar H, Darsinou M, Blokzijl-Franke S, Lenn T, Luscombe NM, Cuda G, Gaspari M, Saiardi A, Riccio A, Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity. Cell Rep, 34(8):108778(2021) [pubmed]

submitter keyword: Protein complex, IMPA1, HuD, Ago2

Marco Gaspari
contact affiliation	Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, Italy
contact email	gaspari@unicz.it
lab head
Marco Gaspari
contact affiliation	University of Catanzaro
contact email	gaspari@unicz.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023586

PRIDE project URI

• PRIDE
  1. PXD023586
     1. Label: PRIDE project
     2. Name: AP-MS of helicase Upf1 in PC12 cells