PXD023577

PXD023577 is an original dataset announced via ProteomeXchange.

Title	Deciphering the architecture and interactome of the spliceosomal C complex using in vivo chemical crosslinking
Description	We used in vivo PIR-based crosslinking to assess specific regions of interaction and protein structure of the spliceosomal C complex components hnRNPA1, A2/B1, and C, and the RALY protein. Our results indicate the interaction of proteins of the C complex via domains regions and conserved motifs, posing as evidence of a coordinated action of known regulatory sequences of RBPs. Moreover, the crosslinking information helped to model some of the RBPs, being complementary to other structural methods available.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:34:36.643.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Helisa Wippel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-14 00:11:21	ID requested
1	2022-10-13 15:36:14	announced
⏵ 2	2023-11-14 07:34:38	announced	2023-11-14: Updated project metadata.

Wippel HH, Fioramonte M, Chavez JD, Bruce JE, Deciphering the architecture and interactome of hnRNP proteins and enigmRBPs. Mol Omics, 17(4):503-516(2021) [pubmed]

submitter keyword: nuclear hnRNPs, chemical cross-linking, splicing,RNA-binding proteins, protein interaction reporter, interactome

James Bruce
contact affiliation	Bruce Lab, Genome Sciences, University of Washington
contact email	jimbruce@uw.edu
lab head
Helisa Wippel
contact affiliation	University of Washington
contact email	hwippel@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD023577

PRIDE project URI

• PRIDE
  1. PXD023577
     1. Label: PRIDE project
     2. Name: Deciphering the architecture and interactome of the spliceosomal C complex using in vivo chemical crosslinking