PXD023571

PXD023571 is an original dataset announced via ProteomeXchange.

Title	Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages
Description	PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is involved in both chromosome segregation during mitotic exit, and potentially establishment of cell polarity during zygote development in the mosquito midgut, suggesting that chemical inhibitors of PP1 may be explored for blocking parasite transmission
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-09_16:09:23.111.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023571
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-13 03:00:48	ID requested
⏵ 1	2021-09-09 16:09:24	announced

Zeeshan M, Pandey R, Subudhi AK, Ferguson DJP, Kaur G, Rashpa R, Nugmanova R, Brady D, Bottrill AR, Vaughan S, Brochet M, Bollen M, Pain A, Holder AA, Guttery DS, Tewari R, Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages. Commun Biol, 4(1):760(2021) [pubmed]

submitter keyword: Phosphatase, Plasmodium, sexual stage, cell division, polarity, kinetochore, Warwick-RTP

Dr. Rita Tewari
contact affiliation	School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham, NG7 2UH
contact email	rita.tewari@nottingham.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023571
     1. Label: PRIDE project
     2. Name: Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages