PXD023567

PXD023567 is an original dataset announced via ProteomeXchange.

Title	Reflux of Endoplasmic Reticulum proteins to the cytosol yields inactivation of tumor suppressors
Description	In the past decades many studies reported the presence of Endoplasmic Reticulum (ER) resident proteins in the cytosol. However the mechanisms by which these proteins relocate and whether they exert cytosolic functions remain unknown. We found that select ER luminal proteins accumulate in the cytosol of glioblastoma cells isolated from mouse and human tumors. In cultured cells, ER protein reflux to the cytosol occurs upon ER proteostasis perturbation. As such we investigated whether refluxed proteins gain new functions in the cytosol, a mechanism that could provide advantage to tumor cells. Using the ER luminal protein AGR2 as a proof of concept, we showed that it is refluxed to the cytosol where it binds and inhibits the tumor suppressor p53. We named this phenomenon ER to Cytosol Signaling (ERCYS) as an ER surveillance mechanism conserved in Eukaryotes to relieve the ER from its contents upon stress that has evolved to provide selective advantage to tumor cells through gain-of-cytosolic functions.
HostingRepository	PRIDE
AnnounceDate	2021-05-03
AnnouncementXML	Submission_2021-05-03_11:35:36.220.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Negroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	glycosylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-13 02:28:15	ID requested
⏵ 1	2021-05-03 11:35:36	announced

Dataset with its publication pending

submitter keyword: Endoplasmic Reticulum, UPR, tumor cells

Eric Chevet
contact affiliation	Centre Eugène Marquis - INSERM U1242, University of Rennes, France
contact email	eric.chevet1@gmail.com
lab head
Luc Negroni
contact affiliation	CNRS
contact email	luc.negroni@igbmc.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023567
     1. Label: PRIDE project
     2. Name: Reflux of Endoplasmic Reticulum proteins to the cytosol yields inactivation of tumor suppressors