PXD023565

PXD023565 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analyses reveal that HIV-1 alters CD4 T cell immunometabolism to fuel virus replication
Description	Human Immunodeficiency Virus type-1 (HIV-1)-infected individuals show metabolic alterations of CD4 T cells through unclear mechanisms with undefined consequences. We analyzed the transcriptome of CD4 T cells from HIV-1 patients and revealed that elevated oxidative phosphorylation (OXPHOS) pathway is associated with poor outcomes. Inhibition of OXPHOS by the FDA-approved drug metformin, which targets mitochondrial respiratory chain complex I, suppresses HIV-1 replication in human CD4 T cells and humanized mice. In patients, HIV-1 peak viremia positively correlates with the expression of NLRX1, a mitochondrial innate immune receptor. Quantitative proteomics and metabolic analyses reveal that NLRX1 enhances OXPHOS and glycolysis during HIV-1-infection of CD4 T cells to promote viral replication. At the mechanistic level, HIV infection induces the association of NLRX1 with the mitochondrial protein, FASTKD5, to promote the expression of mitochondrial respiratory complex components. This study uncovers the OXPHOS pathway in CD4 T cells as a target for HIV-1 therapy.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_13:34:53.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-13 02:28:13	ID requested
⏵ 1	2022-02-16 13:34:54	announced

Guo H, Wang Q, Ghneim K, Wang L, Rampanelli E, Holley-Guthrie E, Cheng L, Garrido C, Margolis DM, Eller LA, Robb ML, Sekaly RP, Chen X, Su L, Ting JP, T cell immunometabolism to fuel virus replication. Nat Immunol, 22(4):423-433(2021) [pubmed]

submitter keyword: HIV-1, Virus replication, CD4 T cell, NLRX1, FASTKD5, Oxidative phosphorylation

Jenny PY Ting
contact affiliation	William R. Kenan,JR. distinguished professor, Genetics co-director, Inflammatory diseases institute director, Center for translational immunology,University of North Carolina at Chapel Hill
contact email	jenny_ting@med.unc.edu
lab head
Li Wang
contact affiliation	Postdoctoral Research Associate
contact email	liwang@email.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD023565

PRIDE project URI

• PRIDE
  1. PXD023565
     1. Label: PRIDE project
     2. Name: Multi-omics analyses reveal that HIV-1 alters CD4 T cell immunometabolism to fuel virus replication