PXD023559

PXD023559 is an original dataset announced via ProteomeXchange.

Title	The zinc finger antiviral protein ZAP destabilises viral transcripts and restricts human cytomegalovirus
Description	Interferon-stimulated gene products (ISGs) play a crucial role in early infection control. The ISG zinc finger CCCH-type antiviral protein 1 (ZAP/ZC3HAV1) antagonises several RNA viruses by binding to CG-rich RNA sequences, whereas its effect on DNA viruses is largely unknown. Here, we decipher the role of ZAP in the context of human cytomegalovirus (HCMV) infection, a β-herpesvirus that is associated with high morbidity in immunosuppressed individuals and newborns. We show that expression of the two major isoforms of ZAP, the long (ZAP-L) and short (ZAP-S), is induced during HCMV infection and that both negatively affect HCMV replication. Transcriptome and proteome analyses demonstrated that the expression of ZAP decelerates the progression of HCMV infection. SLAM-sequencing revealed that ZAP restricts HCMV at early stages of infection by destabilising a distinct subset of viral transcripts with low CG content. In summary, this report provides evidence of an important antiviral role for ZAP in host defense against HCMV infection and highlights its differentiated function during DNA virus infection.
HostingRepository	PRIDE
AnnounceDate	2021-04-22
AnnouncementXML	Submission_2021-04-22_13:30:15.735.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Urban
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-13 00:04:16	ID requested
⏵ 1	2021-04-22 13:30:16	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, proteomics, ISG, ZAP, antiviral, interferon, human cytomegalovirus, innate immunity

Andreas Pichlmair
contact affiliation	Institute of Virology, Technische Universität München, Germany
contact email	andreas.pichlmair@tum.de
lab head
Christian Urban
contact affiliation	Institute of Virology, Technical University Munich
contact email	c.urban@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023559
     1. Label: PRIDE project
     2. Name: The zinc finger antiviral protein ZAP destabilises viral transcripts and restricts human cytomegalovirus