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PXD023557 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlea novel SDS-based sample preparation for proteomics
DescriptionSodium dodecyl sulfate (SDS) is widely used in sample preparation for proteomic research. However, SDS is incompatible with liquid chromatography and electrospray ionization (ESI). SDS depletion is therefore required ahead of liquid chromatography coupled with mass spectrometry (LC-MS) analysis. Most of current SDS removal strategies are time-consuming, laborious and low reproducibility. Here, we describe a method, SDS-cyclodextrin assisted sample preparation (SCASP), by which cyclodextrin can bind to SDS and form cyclodextrins-SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SCASP is a simple, fast and robust SDS-based sample preparation method for proteomics application.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChuan-Qi Zhong
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600; Bruker Daltonics amaZon series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-12 23:25:00ID requested
12021-01-12 23:25:45announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SDS, sample preparation, cyclodextin
Contact List
Chuan-Qi Zhong
contact affiliationXiamen University
contact emailzhongcq@xmu.edu.cn
lab head
Chuan-Qi Zhong
contact affiliationXiamen University
contact emailzhongcq@xmu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI