PXD023557

PXD023557 is an original dataset announced via ProteomeXchange.

Title	a novel SDS-based sample preparation for proteomics
Description	Sodium dodecyl sulfate (SDS) is widely used in sample preparation for proteomic research. However, SDS is incompatible with liquid chromatography and electrospray ionization (ESI). SDS depletion is therefore required ahead of liquid chromatography coupled with mass spectrometry (LC-MS) analysis. Most of current SDS removal strategies are time-consuming, laborious and low reproducibility. Here, we describe a method, SDS-cyclodextrin assisted sample preparation (SCASP), by which cyclodextrin can bind to SDS and form cyclodextrins-SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SCASP is a simple, fast and robust SDS-based sample preparation method for proteomics application.
HostingRepository	iProX
AnnounceDate	2021-03-02
AnnouncementXML	Submission_2021-03-02_21:50:18.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan-Qi Zhong
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600; Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-12 23:25:00	ID requested
1	2021-01-12 23:25:45	announced
⏵ 2	2021-03-02 21:50:18	announced	2021-03-03: Update publication information.

Gan G, Xu X, Chen X, Zhang XF, Wang J, Zhong CQ, SCASP: A Simple and Robust SDS-Aided Sample Preparation Method for Proteomic Research. Mol Cell Proteomics, 20():100051(2021) [pubmed]

submitter keyword: SDS, sample preparation, cyclodextin

Chuan-Qi Zhong
contact affiliation	Xiamen University
contact email	zhongcq@xmu.edu.cn
lab head
Chuan-Qi Zhong
contact affiliation	Xiamen University
contact email	zhongcq@xmu.edu.cn
dataset submitter

iProX dataset URI