PXD023546

PXD023546 is an original dataset announced via ProteomeXchange.

Title	Renal Cell Carcinoma (Renca) Extracellular Vesicles under Hypoxia and Normoxia
Description	Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles and provide a universal molecular cell-cell communication system. Hypoxia is a typical condition in solid tumors, and cancer-derived EVs support growth and invasion of tissues by tumor cells. EVs were purified from cell culture medium by ultracentrifugation followed by size exclusion chromatography with Exo-spin™ columns (Cell Guidance Systems Ltd). We performed proteomic analysis of five hypoxia/normoxia pairs of EV samples; each of these replicates for either hypoxia or normoxia was analyzed in duplicates. Obtained data were compared with proteomics analysis of corresponding cell culture media supernatants (SN) after EV removal with 100 000 g ultracentrifugation. We found that exposure of tumor cells to hypoxia (1% oxygen) induced EV secretion, altered EV sizes, and led to notable changes in the EV protein cargo in comparison to normoxia.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_13:05:14.446.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023546
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Anatoliy Samoylenko
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-12 06:25:07	ID requested
⏵ 1	2022-02-16 13:05:15	announced

Samoylenko A, K, ö, gler M, Zhyvolozhnyi A, Makieieva O, Bart G, Andoh SS, Roussey M, Vainio SJ, Hiltunen J, Time-gated Raman spectroscopy and proteomics analyses of hypoxic and normoxic renal carcinoma extracellular vesicles. Sci Rep, 11(1):19594(2021) [pubmed]

submitter keyword: Mouse, Kidney, Carcinoma, Extracellular Vesisles, LC-MS

Seppo Vainio
contact affiliation	Faculty of Biochemistry and Molecular Medicine, Disease Networks Research Unit, Laboratory of Developmental Biology, Kvantum Institute, Infotech Oulu, University of Oulu, Oulu, Finland
contact email	seppo.vainio@oulu.fi
lab head
Anatoliy Samoylenko
contact affiliation	University of Oulu
contact email	anatoliy.samoylenko@oulu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD023546

PRIDE project URI

• PRIDE
  1. PXD023546
     1. Label: PRIDE project
     2. Name: Renal Cell Carcinoma (Renca) Extracellular Vesicles under Hypoxia and Normoxia