PXD023538

PXD023538 is an original dataset announced via ProteomeXchange.

Title	C4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected Vero E6 cells treated with protease inhibitors (Enriched)
Description	Many events in viral infection are regulated through post-translational modification. In the case of positive-sense RNA viruses such as SARS-CoV-2 which encodes two viral proteases, proteolytic cleavage of both viral and cellular proteins is crucial for productive viral infection. In this work we study proteolysis in the context of SARS-CoV-2 infection of Vero E6 cells, treated with calpeptin or camostat at 12h post-infection and harvested at 24h post-infection. Our goal was Neo-N-termini and lysine residues were labelled at the protein level with TMTpro reagents prior to tryptic digestion. For unenriched samples, the trypsin-digested material was then fractionated offline, and analysed by LC-MS/MS for the study of total protein abundance over the infection timecourse. For enriched samples, unblocked neo-N-termini generated by tryptic digested were labelled with undecanal and depleted, yielding a sample enriched for blocked neo-N-termini (TMTpro, N-terminal acetylation, and pyroGlu). The enriched samples were similarly fractionated offline and analysed by LC-MS/MS. Please note this is one of several linked datasets.
HostingRepository	PRIDE
AnnounceDate	2021-07-30
AnnouncementXML	Submission_2021-07-29_21:08:14.009.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ed Emmott
SpeciesList	scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-12 01:32:58	ID requested
⏵ 1	2021-07-29 21:08:14	announced

Dataset with its publication pending

ProteomeXchange project tag: Covid-19

submitter keyword: SARS-CoV-2, Vero E6, COVID-19, N-terminomics, TMTpro

Edward Emmott
contact affiliation	Centre for Proteome Research, University of Liverpool, Liverpool, UK
contact email	e.emmott@liverpool.ac.uk
lab head
Ed Emmott
contact affiliation	Centre for Proteome Research, University of Liverpool
contact email	e.emmott@liverpool.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD023538

PRIDE project URI

• PRIDE
  1. PXD023538
     1. Label: PRIDE project
     2. Name: C4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected Vero E6 cells treated with protease inhibitors (Enriched)