PXD023537
PXD023537 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | PRM quantification of XRCC4 and Xkr4 peptides that were immunoprecipitated with SPOT-Xkr4-Flag |
Description | Several selected peptides of human XRCC4 and mouse Xkr4 were measured by PRM. LC-MS/MS analysis was performed on an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer through a nanoelectrospray ion source (Thermo Fisher Scientific). Targeted MS/MS scans were acquired by a time-scheduled inclusion list at a resolution of 70,000, an isolation window of 4.0 m/z, a maximum injection time of 2 sec, and a normalized collision energy of 27. Time alignment and relative quantification of the transitions were performed with PinPoint version 1.4 (Thermo Fisher Scientific). |
HostingRepository | jPOST |
AnnounceDate | 2021-01-12 |
AnnouncementXML | Submission_2022-09-18_03:14:57.090.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hidetaka Kosako |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-01-11 23:57:18 | ID requested | |
1 | 2021-01-11 23:57:20 | announced | |
2 | 2021-02-04 16:48:14 | announced | 2021-02-04: Updated FTP location. |
3 | 2021-03-17 02:08:16 | announced | 2021-03-17: Updated PubMed. |
⏵ 4 | 2022-09-18 03:14:57 | announced | 2022-09-18: Updated FTP location. |
Publication List
Maruoka M, Zhang P, Mori H, Imanishi E, Packwood DM, Harada H, Kosako H, Suzuki J, Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol Cell, 81(7):1397-1410.e9(2021) [pubmed] |
Keyword List
submitter keyword: SPOT-Trap, PRM |
Contact List
Hidetaka Kosako | |
---|---|
lab head | |
Hidetaka Kosako | |
contact affiliation | Tokushima University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001058/ |