PXD023537

PXD023537 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	PRM quantification of XRCC4 and Xkr4 peptides that were immunoprecipitated with SPOT-Xkr4-Flag
Description	Several selected peptides of human XRCC4 and mouse Xkr4 were measured by PRM. LC-MS/MS analysis was performed on an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer through a nanoelectrospray ion source (Thermo Fisher Scientific). Targeted MS/MS scans were acquired by a time-scheduled inclusion list at a resolution of 70,000, an isolation window of 4.0 m/z, a maximum injection time of 2 sec, and a normalized collision energy of 27. Time alignment and relative quantification of the transitions were performed with PinPoint version 1.4 (Thermo Fisher Scientific).
HostingRepository	jPOST
AnnounceDate	2021-01-12
AnnouncementXML	Submission_2022-09-18_03:14:57.090.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-11 23:57:18	ID requested
1	2021-01-11 23:57:20	announced
2	2021-02-04 16:48:14	announced	2021-02-04: Updated FTP location.
3	2021-03-17 02:08:16	announced	2021-03-17: Updated PubMed.
⏵ 4	2022-09-18 03:14:57	announced	2022-09-18: Updated FTP location.

Publication List

Maruoka M, Zhang P, Mori H, Imanishi E, Packwood DM, Harada H, Kosako H, Suzuki J, Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol Cell, 81(7):1397-1410.e9(2021) [pubmed]

Maruoka M, Zhang P, Mori H, Imanishi E, Packwood DM, Harada H, Kosako H, Suzuki J, Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol Cell, 81(7):1397-1410.e9(2021) [pubmed]

Keyword List

submitter keyword: SPOT-Trap, PRM

submitter keyword: SPOT-Trap, PRM

Contact List

Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001058/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001058/