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PXD023537

PXD023537 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePRM quantification of XRCC4 and Xkr4 peptides that were immunoprecipitated with SPOT-Xkr4-Flag
DescriptionSeveral selected peptides of human XRCC4 and mouse Xkr4 were measured by PRM. LC-MS/MS analysis was performed on an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer through a nanoelectrospray ion source (Thermo Fisher Scientific). Targeted MS/MS scans were acquired by a time-scheduled inclusion list at a resolution of 70,000, an isolation window of 4.0 m/z, a maximum injection time of 2 sec, and a normalized collision energy of 27. Time alignment and relative quantification of the transitions were performed with PinPoint version 1.4 (Thermo Fisher Scientific).
HostingRepositoryjPOST
AnnounceDate2021-01-12
AnnouncementXMLSubmission_2022-09-18_03:14:57.090.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHidetaka Kosako
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-11 23:57:18ID requested
12021-01-11 23:57:20announced
22021-02-04 16:48:14announced2021-02-04: Updated FTP location.
32021-03-17 02:08:16announced2021-03-17: Updated PubMed.
42022-09-18 03:14:57announced2022-09-18: Updated FTP location.
Publication List
Maruoka M, Zhang P, Mori H, Imanishi E, Packwood DM, Harada H, Kosako H, Suzuki J, Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol Cell, 81(7):1397-1410.e9(2021) [pubmed]
Keyword List
submitter keyword: SPOT-Trap, PRM
Contact List
Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliationTokushima University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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