PXD023510

PXD023510 is an original dataset announced via ProteomeXchange.

Title	Methylation of Q105 on histone H2A is part of a dynamic regulatory mechanism integrating metabolism with ribosome biogenesis through recruitment of Nhp2
Description	Ribosome biogenesis is an essential process within cells that requires integration of extracellular cues such as metabolic states through signaling with transcriptional regulation and maintenance of accessible chromatin at the ribosomal DNA. Here, we demonstrate that the recently identified histone modification, methylation of H2AQ105 is an integral part of a dynamic chromatin network at the rDNA locus. Its deposition depends on a functional mTor signaling pathway and acetylation of histone H3 at position K56, thus integrating signals from cell cycle and proliferative states. Furthermore, we identify a first epigenetic reader of this modification. The ribonucleoprotein Nhp2 specifically recognizes methylation H2AQ105me. Based on functional and proteomic data we suggest that Nhp2 functions as an adapter to bridge chromatin and components of the small subunit processome and might help to efficiently coordinate transcription of rRNA with its post-transcriptional processing.
HostingRepository	PRIDE
AnnounceDate	2021-08-06
AnnouncementXML	Submission_2021-08-05_22:01:52.030.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-11 07:15:44	ID requested
⏵ 1	2021-08-05 22:01:52	announced

Dataset with its publication pending

submitter keyword: SILAC, LFQ, IP

Peter Tessarz
contact affiliation	Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, 50931 Cologne, Germany Cologne Excellence Cluster on Stress Responses in ageing-associated Diseases (CECAD), University of Cologne, Joseph-Stelzmann-Str. 26, 50931 15Cologne, Germany
contact email	ptessarz@age.mpg.de
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023510
     1. Label: PRIDE project
     2. Name: Methylation of Q105 on histone H2A is part of a dynamic regulatory mechanism integrating metabolism with ribosome biogenesis through recruitment of Nhp2