PXD023489

PXD023489 is an original dataset announced via ProteomeXchange.

Title	Proteomic profile of saliva in Parkinson’s disease patients.
Description	Parkinson’s disease (PD) is a progressive neurodegenerative disorder. Lewy pathology involves numerous organs. Submandibular glands are affected in about 75% of in vivo cases, with higher rates of positive findings in post-mortem histopathological studies. Salivary microbiome composi-tion in PD patients is also different than in healthy patients. Therefore, we hypothesize that saliva may serve as a potential source of biomarkers of Parkinson’s disease. To evaluate and compare the salivary proteome of PD patients and healthy controls (HC). Salivary samples from 39 subjects (24 PD patients mean age 61.6 8.2 and 15 HC mean age 60.96.7) were used. Saliva was collected 30 minutes after rinsing mouth with tap water, in the morning hours, using RNA-Pro-Sal kits. Samples were immediately frozen at -80oC after collection. Label-free LC-MS/MS mass spectrometry was performed to comprehensively characterize the proteome of the saliva. IPA analysis of upstream inhibitors was performed. A total of 530 proteins and peptides were identified. We observed significantly lower concentrations of S100-A16, ARP2/3 and VPS4B in PD group vs controls. Additional IPA analysis indicated PD98059 to be most significantly activated and beta-estradiol most significantly inhibited upstream regulators. Conclusions: Very limited data on the salivary proteome of PD subjects is available. The results of our analysis indicate that the salivary proteome of PD patients might be different than that of healthy controls. We observed significantly lower concentrations of proteins involved in inflammatory processes, exosome formation and adipose tissue formation among others. Upstream regulator analysis indicated several proteins previously associated with neurodegeneration to drive the differential protein expression in saliva. The main limitations of the study are variability of expression of proteins between subjects in the two groups and variations caused by external factors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:40:35.056.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023489
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ewa Sitkiewicz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-08 06:18:54	ID requested
1	2021-09-09 16:06:20	announced
⏵ 2	2023-11-14 07:40:37	announced	2023-11-14: Updated project metadata.

Figura M, Sitkiewicz E, Ś, widerska B, Milanowski Ł, Szlufik S, Koziorowski D, Friedman A, Proteomic Profile of Saliva in Parkinson's Disease Patients: A Proof of Concept Study. Brain Sci, 11(5):(2021) [pubmed]

10.6019/PXD023489;

submitter keyword: LC-MS-MS,Parkinson disease, salivary proteom

Michał Dadlez
contact affiliation	Institute of Biochemistry and Biophysics, PAN, Pawinskiego 5a, 02-109, Warsaw, Poland
contact email	michald@ibb.waw.pl
lab head
Ewa Sitkiewicz
contact affiliation	Mass Spectrometry Laboratory Institute of Biochemistry and Biophysics Polish Academy of Sciences
contact email	ewa@ibb.waw.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023489
     1. Label: PRIDE project
     2. Name: Proteomic profile of saliva in Parkinson’s disease patients.