PXD023484

PXD023484 is an original dataset announced via ProteomeXchange.

Title	Iron deficiency and recovery in yeast: a quantitative proteomics approach
Description	A model used to study iron deficiency is yeast, Saccharomyces cerevisiae. Here, we used quantitative (phospho)proteomics to explore the early (4h and 6h) and late (12h) response to ID. We showed that metabolic pathways like the Krebs cycle, amino acid and ergosterol biosynthesis were affected by ID. In addition, during the late response, several proteins related to the ubiquitin-proteasome system and autophagy were upregulated. We also explored the proteomic changes during a recovery period after 12h of ID. We found that several proteins recovered their steady-state levels, but some others such as cytochromes did not recover during the time tested. Our results highlight the complex proteome changes occurring during ID and recovery. This study constitutes a valuable dataset for researchers interested in iron biology, offering a temporal proteomic dataset for ID, as well as a compendium the proteomic changes associated with episodes of iron recovery
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_15:53:43.692.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jose Navarrete Perea
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-08 05:21:48	ID requested
⏵ 1	2022-05-19 15:53:44	announced

Navarrete-Perea J, Guerra-Moreno A, Van Vranken J, Isasa M, Paulo JA, Gygi SP, Iron Deficiency and Recovery in Yeast: A Quantitative Proteomics Approach. J Proteome Res, 20(5):2751-2761(2021) [pubmed]

submitter keyword: SL-TMT, Orbitrap Fusion, Iron-sulfur cluster, ubiquitin-proteasome system, nutritional deficiency

Steven P.Gygi
contact affiliation	Professor, Dept. Cell Biology Director, Taplin Biological MS Facility
contact email	Steven_Gygi@hms.harvard.edu
lab head
Jose Navarrete Perea
contact affiliation	Harvard Medical School
contact email	Jose_Navarrete-Perea@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023484
     1. Label: PRIDE project
     2. Name: Iron deficiency and recovery in yeast: a quantitative proteomics approach