PXD023474

PXD023474 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis in the study of the mitochondrial supercomplex assembly
Description	Mitochondrial supercomplexes are functionally important, but how supercomplexes being assembled is not known. Mitochondrial Complex I has an important role in the assembly of supercomplex. In this study, we generated a series of cell model with shRNA mediated gene silencing or CRISPR-Cas9 gene knockout technology. We performed a series of mitochondrial proteome analyses in different cell lines with disruption of gene that encodes subunit of Complex I. Also, we performed Co-IP of exogenous TIMMDC1, which was an assembly factor of Complex I.
HostingRepository	iProX
AnnounceDate	2021-01-08
AnnouncementXML	Submission_2022-09-21_23:10:48.519.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xianglai Ye
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-08 04:06:07	ID requested
⏵ 1	2022-09-21 23:10:49	announced

Fang H, Ye X, Xie J, Li Y, Li H, Bao X, Yang Y, Lin Z, Jia M, Han Q, Zhu J, Li X, Zhao Q, Yang Y, Lyu J, A membrane arm of mitochondrial complex I sufficient to promote respirasome formation. Cell Rep, 35(2):108963(2021) [pubmed]

submitter keyword: mitochondria, supercomplex assembly, mitochondrial proteomics, Co-IP

Jianxin Lyu
contact affiliation	Wenzhou Medical University
contact email	jxlu313@163.com
lab head
Xianglai Ye
contact affiliation	Wenzhou Medical University
contact email	xlye96@outlook.com
dataset submitter

iProX dataset URI