PXD023472 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation |
Description | CSA and CSB proteins are key players in transcription-coupled nucleotide excision repair (TC-NER) pathway that removes UV-induced DNA lesions from the transcribed strands of expressed genes. Additionally, CS proteins play relevant but still elusive roles in other cellular pathways whose alteration may explain neurodegeneration and progeroid features in Cockayne syndrome (CS). Here we identify a CSA-dependent chromatin-associated protein complex that modulates rRNA transcription. Besides RNA polymerase I (RNAP1) and specific ribosomal proteins (RPs), the CSA complex includes ferrochelatase (FECH), a well-known mitochondrial enzyme whose deficiency causes erythropoietic protoporphyria (EPP). Impairment of either CSA or FECH functionality leads to reduced RNAP1 occupancy on rDNA promoter that is associated to reduced transcription when CSA is affected and abnormal ribosomal transcript accumulation after FECH silencing. Accordingly, primary fibroblasts from CS and EPP fibroblasts show opposed amount of total rRNAs. After cell irradiation with UV light, CSA triggers the dissociation of the CSA-FECH-RNAP1-RPs complex from the chromatin while it stabilizes its binding to FECH. Besides disclosing a function for FECH within nucleoli, this study sheds light on the still unknown mechanisms through which CSA modulates rRNA transcription. |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-11 |
AnnouncementXML | Submission_2021-10-10_23:13:44.225.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Claudia Landi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | ultraflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-08 03:02:24 | ID requested | |
⏵ 1 | 2021-10-10 23:13:44 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: CS proteins, FECH, nucleolus, UV-induced DNA damage, RNAP1 transcription |
Contact List
Donata Orioli |
contact affiliation | Istituto di Genetica Molecolare CNR, Pavia 27100, Italy |
contact email | orioli@igm.cnr.it |
lab head | |
Claudia Landi |
contact affiliation | University of Siena |
contact email | landi35@unisi.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023472
- Label: PRIDE project
- Name: Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation