PXD023472

PXD023472 is an original dataset announced via ProteomeXchange.

Title	Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation
Description	CSA and CSB proteins are key players in transcription-coupled nucleotide excision repair (TC-NER) pathway that removes UV-induced DNA lesions from the transcribed strands of expressed genes. Additionally, CS proteins play relevant but still elusive roles in other cellular pathways whose alteration may explain neurodegeneration and progeroid features in Cockayne syndrome (CS). Here we identify a CSA-dependent chromatin-associated protein complex that modulates rRNA transcription. Besides RNA polymerase I (RNAP1) and specific ribosomal proteins (RPs), the CSA complex includes ferrochelatase (FECH), a well-known mitochondrial enzyme whose deficiency causes erythropoietic protoporphyria (EPP). Impairment of either CSA or FECH functionality leads to reduced RNAP1 occupancy on rDNA promoter that is associated to reduced transcription when CSA is affected and abnormal ribosomal transcript accumulation after FECH silencing. Accordingly, primary fibroblasts from CS and EPP fibroblasts show opposed amount of total rRNAs. After cell irradiation with UV light, CSA triggers the dissociation of the CSA-FECH-RNAP1-RPs complex from the chromatin while it stabilizes its binding to FECH. Besides disclosing a function for FECH within nucleoli, this study sheds light on the still unknown mechanisms through which CSA modulates rRNA transcription.
HostingRepository	PRIDE
AnnounceDate	2021-10-11
AnnouncementXML	Submission_2021-10-10_23:13:44.225.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudia Landi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-08 03:02:24	ID requested
⏵ 1	2021-10-10 23:13:44	announced

Dataset with its publication pending

submitter keyword: CS proteins, FECH, nucleolus, UV-induced DNA damage, RNAP1 transcription

Donata Orioli
contact affiliation	Istituto di Genetica Molecolare CNR, Pavia 27100, Italy
contact email	orioli@igm.cnr.it
lab head
Claudia Landi
contact affiliation	University of Siena
contact email	landi35@unisi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD023472

PRIDE project URI

• PRIDE
  1. PXD023472
     1. Label: PRIDE project
     2. Name: Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation