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PXD023472

PXD023472 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation
DescriptionCSA and CSB proteins are key players in transcription-coupled nucleotide excision repair (TC-NER) pathway that removes UV-induced DNA lesions from the transcribed strands of expressed genes. Additionally, CS proteins play relevant but still elusive roles in other cellular pathways whose alteration may explain neurodegeneration and progeroid features in Cockayne syndrome (CS). Here we identify a CSA-dependent chromatin-associated protein complex that modulates rRNA transcription. Besides RNA polymerase I (RNAP1) and specific ribosomal proteins (RPs), the CSA complex includes ferrochelatase (FECH), a well-known mitochondrial enzyme whose deficiency causes erythropoietic protoporphyria (EPP). Impairment of either CSA or FECH functionality leads to reduced RNAP1 occupancy on rDNA promoter that is associated to reduced transcription when CSA is affected and abnormal ribosomal transcript accumulation after FECH silencing. Accordingly, primary fibroblasts from CS and EPP fibroblasts show opposed amount of total rRNAs. After cell irradiation with UV light, CSA triggers the dissociation of the CSA-FECH-RNAP1-RPs complex from the chromatin while it stabilizes its binding to FECH. Besides disclosing a function for FECH within nucleoli, this study sheds light on the still unknown mechanisms through which CSA modulates rRNA transcription.
HostingRepositoryPRIDE
AnnounceDate2021-10-11
AnnouncementXMLSubmission_2021-10-10_23:13:44.225.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterClaudia Landi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
Instrumentultraflex
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-08 03:02:24ID requested
12021-10-10 23:13:44announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CS proteins, FECH, nucleolus, UV-induced DNA damage, RNAP1 transcription
Contact List
Donata Orioli
contact affiliationIstituto di Genetica Molecolare CNR, Pavia 27100, Italy
contact emailorioli@igm.cnr.it
lab head
Claudia Landi
contact affiliationUniversity of Siena
contact emaillandi35@unisi.it
dataset submitter
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Dataset FTP location
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