PXD023460

PXD023460 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of of the exoproteome of a clathrin mutant
Description	Fungi are the most important plant pathogens on agricultural and horticultural crops. Study of these pathogens remains essential to understand pathogenic process and control plant diseases. In this context, a random insertional mutagenesis strategy, based on the Agrobacterium tumefaciens-mediated transformation (ATMT), was used to create a mutant library of the grey mould fungus Botrytis cinerea. A nonpathogenic mutant altered in the expression of a gene encoding clathrin heavy chain was selected. Clathrin heavy chain is well conserved from yeast to human and is described to be mainly involved in intracellular coat vesicles formation for endocytosis and post-Golgi trafficking of proteins. The characterization of the mutant was performed to precise the role of this protein in the infectious process of this typical necrotrophic fungus. Using a proteomic approach, a secretion defect of numerous proteins including known virulence factors, as Plant Cell Wall Degrading Enzymes and elicitors was observed. This study demonstrated for the first time the essential role of clathrin in the infectious process of a fungal pathogen.
HostingRepository	PRIDE
AnnounceDate	2021-06-11
AnnouncementXML	Submission_2021-06-10_22:08:19.205.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Botrytis cinerea B05.10; NCBI TaxID: 332648;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-07 08:51:32	ID requested
⏵ 1	2021-06-10 22:08:19	announced

Dataset with its publication pending

submitter keyword: phytopathogneic fungus, clathrin mutant, virulence, quantitative proteome,

Nathalie Poussereau
contact affiliation	Functional genomics of plant pathogenic fungi UMR 5240 Microbiology Adaptation Pathogeny CNRS-UCB-INSA-Bayer CropScience BAYER SAS, Bayer CropScience 14 Impasse Pierre Baizet, BP 99163 69263 Lyon Cedex 09, France
contact email	nathalie.poussereau@univ-lyon1.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD023460

PRIDE project URI

• PRIDE
  1. PXD023460
     1. Label: PRIDE project
     2. Name: Proteomic analysis of of the exoproteome of a clathrin mutant