PXD023457 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of U251 MG glioblastoma cells in the presence and absence of α-fodrin |
Description | The cortical cytoskeleton comprises mainly of the spectrin family of proteins. Spectrin and fodrin are members of this family. They support the plasma membrane. Fodrin is expressed in most tissues apart from the erythrocytes. Fodrin has been shown to be important in a wide variety of functions such as signal transduction, cardiac and brain development, etc. Through our studies it was understood that fodrin not only serves as a cortical supporting protein, but it also interacts with γ-tubulin and inhibits its microtubule nucleation activity. To gain a comprehensive view on the diverse functions of fodrin it was imperative to look into the downstream effectors of this protein in an unbiased fashion. Hence, we decided to downregulate α-fodrin in glioblastoma cells U-251 MG, and then perform a global protein expression analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:22:14.265.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD023457 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | MAHESH CHANDRAN V R |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | L-methionine sulfone; S-carboxamidomethyl-L-cysteine |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-07 06:37:37 | ID requested | |
1 | 2021-05-24 06:41:29 | announced | |
⏵ 2 | 2024-10-22 05:22:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: α-fodrin,LC-MS,shRNA,U251 MG glioblastoma cells |
Contact List
Dr.Suparna sengupta |
contact affiliation | scientist F Rajiv Gandhi Centre for Biotechnology |
contact email | ssengupta@rgcb.res.in |
lab head | |
MAHESH CHANDRAN V R |
contact affiliation | Technician, Mass spectrometry and proteomics core facility, RGCB |
contact email | maheshchandran@rgcb.res.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD023457 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023457
- Label: PRIDE project
- Name: Proteomic analysis of U251 MG glioblastoma cells in the presence and absence of α-fodrin