PXD023457

PXD023457 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of U251 MG glioblastoma cells in the presence and absence of α-fodrin
Description	The cortical cytoskeleton comprises mainly of the spectrin family of proteins. Spectrin and fodrin are members of this family. They support the plasma membrane. Fodrin is expressed in most tissues apart from the erythrocytes. Fodrin has been shown to be important in a wide variety of functions such as signal transduction, cardiac and brain development, etc. Through our studies it was understood that fodrin not only serves as a cortical supporting protein, but it also interacts with γ-tubulin and inhibits its microtubule nucleation activity. To gain a comprehensive view on the diverse functions of fodrin it was imperative to look into the downstream effectors of this protein in an unbiased fashion. Hence, we decided to downregulate α-fodrin in glioblastoma cells U-251 MG, and then perform a global protein expression analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:22:14.265.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023457
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	MAHESH CHANDRAN V R
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfone; S-carboxamidomethyl-L-cysteine
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-07 06:37:37	ID requested
1	2021-05-24 06:41:29	announced
⏵ 2	2024-10-22 05:22:14	announced	2024-10-22: Updated project metadata.

10.6019/PXD023457;

submitter keyword: α-fodrin,LC-MS,shRNA,U251 MG glioblastoma cells

Dr.Suparna sengupta
contact affiliation	scientist F Rajiv Gandhi Centre for Biotechnology
contact email	ssengupta@rgcb.res.in
lab head
MAHESH CHANDRAN V R
contact affiliation	Technician, Mass spectrometry and proteomics core facility, RGCB
contact email	maheshchandran@rgcb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD023457

PRIDE project URI

• PRIDE
  1. PXD023457
     1. Label: PRIDE project
     2. Name: Proteomic analysis of U251 MG glioblastoma cells in the presence and absence of α-fodrin