PXD023451

PXD023451 is an original dataset announced via ProteomeXchange.

Title	Nucleolar TFIIE plays a role in ribosomal biogenesis and performance
Description	Ribosome biogenesis is a highly energy-demanding process in eukaryotes which requires the concerted action of all three RNA polymerases. In RNA polymerase II transcription, the general transcription factor TFIIH is recruited by TFIIE to the initiation site of protein-coding genes. Distinct mutations in TFIIH and TFIIE give rise to the degenerative disorder trichothiodystrophy (TTD). Here we uncovered an unexpected role of TFIIE in ribosomal RNA synthesis by RNA polymerase I. With high resolution microscopy we detected TFIIE in the nucleolus where TFIIE binds to actively transcribed rDNA. Mutations in TFIIE affects gene-occupancy of RNA polymerase I, rRNA maturation, ribosomal assembly and performance. In consequence, the elevated translational error rate with imbalanced protein synthesis and turnover results in an increase in heat-sensitive proteins. Collectively, mutations in TFIIE – due to impaired ribosomal biogenesis and translational accuracy – lead to a loss of protein homeostasis (proteostasis) which can partly explain the clinical phenotype in TTD.
HostingRepository	PRIDE
AnnounceDate	2021-11-16
AnnouncementXML	Submission_2021-11-16_06:31:56.067.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-07 03:48:50	ID requested
⏵ 1	2021-11-16 06:31:56	announced

Phan T, Maity P, Ludwig C, Streit L, Michaelis J, Tsesmelis M, Scharffetter-Kochanek K, Iben S, Nucleolar TFIIE plays a role in ribosomal biogenesis and performance. Nucleic Acids Res, 49(19):11197-11210(2021) [pubmed]

submitter keyword: Ribosome, RNA polymerase I, TFIIE, translational fidelity, proteostasis

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) Technische Universität München (TUM)
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023451
     1. Label: PRIDE project
     2. Name: Nucleolar TFIIE plays a role in ribosomal biogenesis and performance