PXD023449

PXD023449 is an original dataset announced via ProteomeXchange.

Title	Staphylococcus aureus toxin-antitoxin system
Description	Type I toxin-antitoxin (TA) systems are widespread genetic modules in bacterial genomes. They express toxic peptides whose overexpression leads to growth arrest or cell death, whereas antitoxins regulate the expression of toxins, acting as labile antisense RNAs. The Staphylococcus aureus (S. aureus) genome contains and expresses several functional type I TA systems, but their biological functions remain unclear. Here we addressed and challenged experimentally, by proteomics, if a type I TA system, the SprF1/SprG1 pair, influences the overall gene expression in S. aureus. Deleted and complemented S. aureus strains were analyzed for their proteomes, both intracellular and extracellular, during growth. Comparison of intracellular proteomes between the strains points on SprF1 antitoxin as an agent downregulating protein expression. In the strain naturally expressing the SprG1 toxin, cytoplasmic proteins are excreted into the medium, but this is not due to unspecific cell leakages. Such toxin-driven release of the cytoplasmic proteins may modulate the host inflammatory response that, in turn, may amplify the S. aureus infection spread.
HostingRepository	PRIDE
AnnounceDate	2021-07-05
AnnouncementXML	Submission_2021-07-05_08:59:45.459.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023449
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kinga Chlebicka
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	iodoacetamide derivatized residue
Instrument	Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-07 02:24:26	ID requested
⏵ 1	2021-07-05 08:59:46	announced

Chlebicka K, Bonar E, Suder P, Ostyn E, Felden B, Wladyka B, Pinel-Marie ML, Gene Expression. Genes (Basel), 12(5):(2021) [pubmed]

submitter keyword: S. aureus

toxin-antitoxin system

proteomics

cell death

Benedykt Wladyka
contact affiliation	1Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
contact email	benedykt.wladyka@uj.edu.pl
lab head
Kinga Chlebicka
contact affiliation	Jagielloninan University
contact email	kinga.chlebicka@student.uj.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD023449

PRIDE project URI

• PRIDE
  1. PXD023449
     1. Label: PRIDE project
     2. Name: Staphylococcus aureus toxin-antitoxin system