PXD023438

PXD023438 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Datasets for Mec1/ATR Signaling Upon Hyper-Resection (TMT Supplement)
Description	The Mec1/ATR kinase is crucial for genome maintenance, although the mechanisms by which it controls DNA repair pathways remain elusive. Here we uncovered a role for Mec1/ATR in controlling homologous recombination (HR) factors in response to hyper-resection of DNA ends. Cells lacking RAD9, a checkpoint activator and an inhibitor of resection, exhibit Mec1-dependent hyper-phosphorylation of proteins associated with single strand DNA transactions, including the ssDNA binding protein Rfa2, the translocase/ubiquitin ligase Uls1 and the HR-regulatory Sgs1-Top3-Rmi1 (STR) complex. Extensive Mec1-dependent phosphorylation of the STR complex, mostly on the Sgs1 helicase subunit, promotes an interaction between STR and the DNA repair scaffolding protein Dpb11.
HostingRepository	PRIDE
AnnounceDate	2021-03-10
AnnouncementXML	Submission_2021-03-10_08:18:25.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ethan Sanford
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-06 03:45:57	ID requested
⏵ 1	2021-03-10 08:18:25	announced

Dataset with its publication pending

submitter keyword: SILAC, phosphoproteomics, Sgs1, Mec1, Resection

Ethan Sanford
contact affiliation	Marcus Smolka's Lab, Dept. of Molecular Biology and Genetics, Cornell University
contact email	ejs372@cornell.edu
lab head
Ethan Sanford
contact affiliation	Graduate Student, Smolka Lab, Cornell University
contact email	ejs372@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD023438

PRIDE project URI

• PRIDE
  1. PXD023438
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Datasets for Mec1/ATR Signaling Upon Hyper-Resection (TMT Supplement)