PXD023434

PXD023434 is an original dataset announced via ProteomeXchange.

Title	The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity.
Description	Biogenesis of eukaryotic box C/D snoRNPs initiates co-transcriptionally and requires the action of an assembly machinery including the HSP90/R2TP complex, Rsa1p:Hit1p (NUFIP1:ZNHIT3 in human) heterodimer and the Bcd1 protein (ZNHIT6 in human). We identified genetic interactions between the Rsa1p-encoding gene and genes involved in chromatin organization including RTT106 that codes for the H3-H4 histone chaperone Rtt106p controlling H3K56ac deposition. We show that Bcd1p binds Rtt106p directly. Whereas the interaction does not appear to contribute or interfere with known Bcd1p functions during snoRNP biogenesis, we show that Bcd1p controls the transcription-dependent recruitment of Rtt106p by reducing its association with RNA polymerase II, modulating H3K56ac levels at gene body. We reveal the 3D structures of the free and Rtt106p-bound forms of Bcd1p using NMR and X-ray crystallography, and we studied the interaction by a combination of biophysical and proteomic techniques. Bcd1p interacts with a region in the PH1 domain of Rtt106p that is distinct from the interaction interface between the histone chaperone and histone H3. Our results are evidence for a new protein interaction interface for Rtt106p that controls its transcription-associated activity.
HostingRepository	PRIDE
AnnounceDate	2021-02-22
AnnouncementXML	Submission_2021-04-22_22:44:57.958.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steve Hessmann
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt G2 HDMS; TripleTOF 5600; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-06 02:44:02	ID requested
1	2021-02-21 22:52:08	announced
⏵ 2	2021-04-22 22:44:58	announced	2021-04-23: Updated publication reference for PubMed record(s): 33767140.

Bragantini B, Charron C, Bourguet M, Paul A, Tiotiu D, Roth, é B, Marty H, Terral G, Hessmann S, Decourty L, Chagot ME, Strub JM, Massenet S, Bertrand E, Quinternet M, Saveanu C, Cianf, é, rani S, Labialle S, Manival X, Charpentier B, The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity. Nat Commun, 12(1):1859(2021) [pubmed]

submitter keyword: Saccharomyces cerevisiae, snoRNP assembly, Rsa1p, Bcd1p, histone chaperone Rtt106p, 3D structure, NMR, X-ray, native MS, H/D exchange – MS and cross-linking-MS

Sarah Cianférani
contact affiliation	LSMBO IPHC CNRS
contact email	sarah.cianferani@unistra.fr
lab head
Steve Hessmann
contact affiliation	LSMBO IPHC CNRS
contact email	steve.hessmann2@etu.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023434
     1. Label: PRIDE project
     2. Name: The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity.